Prolactin Rat ELISA

Summary

Prolactin is a hormone of molecular weight ca. 20,000 which is secreted from anterior lobe of pituitary, and enhances the growth of mammary gland and milk secretion in the Mammalian. In studies with animals, it becomes possible to detect abnormalities of hypophysis adenoma, and abnormalities of secretory modulation mechanism and secretory functions by measuring the concentration of blood prolactin.

Research topic

Animal studies

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Storage/Shipping

Storage in the dark and cool place (2 – 10°C).

Calibration Curve

Calibration range

0.78 to 50 ng/ml

References to summary

• Allolio B, Hoeppener A, Leonhardt U, Deuss U, Winkelmann W. Size heterogeneity of immunoreactive prolactin in patients with prolactinoma. Acta Endocrinol (Copenh). 1987 Apr;114 (4):475-82
• Bonhoff A, Vuille JC, Gomez F, Gellersen B. Identification of macroprolactin in a patient with asymptomatic hyperprolactinemia as a stable PRL-IgG complex. Exp Clin Endocrinol Diabetes. 1995;103 (4):252-5
• Brownell J, Wide L, Bergh T, Rasmussen C. CV 205-502: a new long-acting drug for inhibition of prolactin hypersecretion. Clin Endocrinol (Oxf). 1987 Mar;26 (3):321-6
• Fahie-Wilson MN. Detection of macroprolactin causing hyperprolactinemia in commercial assays for prolactin. Clin Chem. 2000 Dec;46 (12):2022-3
• Ferland LH, Houdebine LM, Katoh M, Djiane J, Teyssot B, Dusanter-Fourt I, Kelly PA. The interaction of prolactin with its receptors in target tissues and its mechanism of action. Recent Prog Horm Res. 1984;40:379-439
• Frantz AG. Prolactin. N Engl J Med. 1978 Jan 26;298 (4):201-7
• Frantz AG, Kleinberg DL. Human prolactin: measurement in plasma by in vitro bioassay. J Clin Invest. 1971 Aug;50 (8):1557-68
• Friesen H, Guyda H, Hardy J. The biosynthesis of human growth hormone and prolactin. J Clin Endocrinol Metab. 1970 Dec;31 (6):611-24
• Friesen H, Guyda H, Hwang P. Purification of human prolactin. J Biol Chem. 1972 Apr 10;247 (7):1955-8
• Hwang P, Friesen H. Human prolactin. Annu Rev Med. 1973;24:251-70
• Hwang P, Guyda H, Friesen H. 2,3-diphosphoglycerate affects enzymes of glucose metabolism in red blood cells. Nat New Biol. 1971 Jul 7;232 (27):19-20
• Hwang P, Guyda H, Friesen H. A radioimmunoassay for human prolactin. Proc Natl Acad Sci U S A. 1971 Aug;68 (8):1902-6
• Iwasiow BM, Shiu RP. Prolactin-inducible proteins in human breast cancer cells. J Biol Chem. 1985 Sep 15;260 (20):11307-13
• Kaplan SL, Grumbach MM, Aubert ML. Heterologous radioimmunoassay for plasma human prolactin (hPRL); values in normal subjects, puberty, pregnancy and in pituitary disorders. Acta Endocrinol (Copenh). 1974 Nov;77 (3):460-76
• Kelly PA, Posner BI, Tsushima T, Friesen HG. Studies of insulin, growth hormone and prolactin binding: ontogenesis, effects of sex and pregnancy. Endocrinology. 1974 Aug;95 (2):532-9
• Linzer DI, Nathans D. Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family. Proc Natl Acad Sci U S A. 1984 Jul;81 (14):4255-9
• McLeod. RM. Regulation of Prolactin Secretion: In Frontiers in Neuroendocrinology (Martin L. and Garrong N.F. eds). Raven Press. 164,1976;
• Myres RP, Forsyth IA. Human prolactin. Evidence obtained by the bioassay of human plasma. J Endocrinol. 1971 Sep;51 (1):157-68
• Ratcliffe JG, Cowden EA, Beastall GH, Ratcliffe WA. Laboratory assessment of prolactin status. Ann Clin Biochem. 1979 May;16 (3):113-21
• Reyes FI, Winter JS, Faiman C. Pituitary-ovarian relationships preceding the menopause. I. A cross-sectional study of serum follice-stimulating hormone, luteinizing hormone, prolactin, estradiol, and progesterone levels. Am J Obstet Gynecol. 1977 Nov 1;129 (5):557-64
• Schneider W, Marcovitz S, Al-Shammari S, Yago S, Chevalier S. Reactivity of macroprolactin in common automated immunoassays. Clin Biochem. 2001 Sep;34 (6):469-73
• Seavey BK, Singh RN, Lewis UJ. Human prolactin: isolation and some properties. Biochem Biophys Res Commun. 1971 Sep;44 (5):1169-76
• Shome B, Parlow AF. Human pituitary prolactin (hPRL): the entire linear amino acid sequence. J Clin Endocrinol Metab. 1977 Nov;45 (5):1112-5
• Sobrinho LG, Arranhado E, Santos MA, Leite V, Cavaco B. Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. J Clin Endocrinol Metab. 1995 Aug;80 (8):2342-6
• Szonyi E, Mason AJ, Ramachandran J, Seeburg PH, Nikolics K. A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone. Nature. 1985 Aug 8-14;316 (6028):511-7
• Tyson JE, Hwang P, Guyda H, Friesen HG. Studies of prolactin secretion in human pregnancy. Am J Obstet Gynecol. 1972 May 1;113 (1):14-20
• Utiger RD, Wilber JF, Daughaday WH, Jacobs LS, Snyder PJ. Increased serum prolactin after administration of synthetic thyrotropin releasing hormone (TRH) in man. J Clin Endocrinol Metab. 1971 Dec;33 (6):996-8

By research topic:

• Animal studies

By molecule:

• Prolactin