Prorenin Human ELISA

Summary

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.

Research topic

Diabetology - Other Relevant Products, Renal disease

Assay format

Direct ELISA, HRP-labelled antibody

Applications

Cell culture medium, Plasma, Serum, Tissue extract

Storage/Shipping

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at – 70°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

Calibration Curve

Calibration range

0.005–1 ng/ml

References to summary

• Deinum J, Derkx FH, Schalekamp MA, Danser AJ. Newly developed renin and prorenin assays and the clinical evaluation of renin inhibitors. J Hypertens. 2008 May;26 (5):928-37
• Ishida Y, Suzuki F, Yoshida A, Hikichi T, Takahashi A, Izumi N, Nagaoka T, Kai K, Mori F, Yokota H. Serum prorenin levels and diabetic retinopathy in type 2 diabetes: new method to measure serum level of prorenin using antibody activating direct kinetic assay. Br J Ophthalmol. 2005 Jul;89 (7):871-3
• Riegger GA, Hense HW, Schalekamp MA, Derkx FH, Danser AH, Schunkert H. Determinants of interindividual variation of renin and prorenin concentrations: evidence for a sexual dimorphism of (pro)renin levels in humans. J Hypertens. 1998 Jun;16 (6):853-62
• Schwartz HC, Wilson DM, Kraemer FB, Luetscher JA, Bryer-Ash M. Increased plasma inactive renin in diabetes mellitus. A marker of microvascular complications. N Engl J Med. 1985 May 30;312 (22):1412-7

By research topic:

• Diabetology - Other Relevant Products
• Renal disease

By molecule:

• Prorenin

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