Prostaglandin D Synthase (Lipocalin-type) Human ELISA

Summary

Lipocalin – type Prostaglandin D synthase (Beta – Trace Protein, BTP) catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/re­laxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM (non rapid eye movement) sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.It has been proposed that the urinary and serum levels may provide a sensitive indicator of renal damage in diabetes mellitus and hypertension. Elevated levels in the coronary circulation may also be associated with angina. Changes in charge and molecular weight microheterogeneity, due to modification of the N-linked oligosaccharides, may be associated with neurodegenerative disease and multiple sclerosis. Detected in meningioma but not in other brain tumors and may be considered a specific cell marker for meningioma.

Features

• European Union and rest of the world: for research use only.
• The total assay time is less than 3 hours
• The kit measures Beta-trace Protein in serum and plasma (EDTA, citrate, heparin).
• Assay format is 96 wells.
• Quality Controls are human serum based. No animal sera are used.
• Standard is purified native protein based.
• Components of the kit are provided ready to use or concentrated.

Research topic

Neural tissue damage markers, Renal disease

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cerebrospinal fluid, Plasma, Serum, Urine

Sample requirements

100 μl/well

Storage/Shipping

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

1 – 40 ng/ml

Limit of detection

0.5 ng/ml

Intra-assay (Within-Run, n=8)

CV = 4.08%

Inter-assay (Run-to-Run, n=6)

CV = 4.19%

Spiking Recovery

92.1%

Dilution Linearity

93.1%

Cross-Reactivity

References to this product

• Snell-Bergeon JK, Rewers M, Hazlett F, Li X, Dazard JE, Chance MR, Maahs DM, Schlatzer D. Novel urinary protein biomarkers predicting the development of microalbuminuria and renal function decline in type 1 diabetes. Diabetes Care. 2012 Mar;35 (3):549-55

References to summary

• Bokenkamp A, Stoffel-Wagner B, Lentze MJ, Buderus S, Schmitt J, Duker G, Schlieber M, Franke I. Beta-trace protein--a marker of kidney function in children: "Original research communication-clinical investigation". Clin Biochem. 2007 Sep;40 (13-14):969-75
• Conradt HS, Nimtz M, Hoffmann A. Molecular characterization of beta-trace protein in human serum and urine: a potential diagnostic marker for renal diseases. Glycobiology. 1997 Jun;7 (4):499-506
• Hayaishi O, Urade Y. Biochemical, structural, genetic, physiological, and pathophysiological features of lipocalin-type prostaglandin D synthase. Biochim Biophys Acta. 2000 Oct 18;1482 (1-2):259-71
• Hayaishi O, Urade Y, Matsu-ura Y, Kijima Y, Seiki K, Oda H, Eguchi N, Eguchi Y. Expression of lipocalin-type prostaglandin D synthase (beta-trace) in human heart and its accumulation in the coronary circulation of angina patients. Proc Natl Acad Sci U S A. 1997 Dec 23;94 (26):14689-94
• Hayaishi O, Urade Y, Oda H, Eguchi N, Murata Y, Saji F, Kimura T, Nobunaga T, Shimoya K, Kubota Y, Kunishige I, Tokugawa Y. Lipocalin-type prostaglandin D synthase in human male reproductive organs and seminal plasma. Biol Reprod. 1998 Feb;58 (2):600-7
• Hoffmann A, Conradt HS, Gross G, Nimtz M, Lottspeich F, Wurster U. Purification and chemical characterization of beta-trace protein from human cerebrospinal fluid: its identification as prostaglandin D synthase.
• Inoue T, Eguchi Y, Matsumoto T, Kijima Y, Kato Y, Ozaki Y, Waseda K, Oda H, Seiki K, Node K, Urade Y. Lipocalin-type prostaglandin D synthase is a powerful biomarker for severity of stable coronary artery disease. Atherosclerosis. 2008 Dec;201 (2):385-91
• Jung K, Althaus H, Akbari A, Matzinger M, Keely E, Clark H, Vollmer I, Sinha P, Lepage N, Priem F, Filler G. Beta-trace protein, cystatin C, beta(2)-microglobulin, and creatinine compared for detecting impaired glomerular filtration rates in children. Clin Chem. 2002 May;48 (5):729-36
• Knoll GA, Lepage N, Filler G, Dinh L, Hussain N, Fergusson D, Doucette S, Akbari A, White CA. A novel equation to estimate glomerular filtration rate using beta-trace protein. Clin Chem. 2007 Nov;53 (11):1965-8
• Sauerbruch T, Gerhardt T, Klehr HU, Klein B, Stoffel-Wagner B, Poge U, Woitas RP. Serum levels of beta-trace protein and its association to diuresis in haemodialysis patients. Nephrol Dial Transplant. 2008 Jan;23 (1):309-14
• Stejskal D, Ochmanova R, Novackova L, Solichova P. Assessment of serum beta-trace protein (BTP) measurement in the prediction of glomerular filtration rate. Comparison with serum cystatin C. Biomed Pap Med Fac Univ Palack. 2006 Jul;150 (1):83-4
• Taniike M, Ozono K, Nakayama M, Urade Y, Wada K, Kagitani-Shimono K, Kanekiyo T, Okabe-Arahori H, Mohri I, Taniguchi H. Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains. Neurosci Lett. 2007 Jun 8;420 (1):39-44
• Umemura S, Urade Y, Seiki K, Oda H, Takagi M, Eguchi Y, Ikeda T, Gomi T, Toya Y, Yamakado M, Uehara Y, Hirawa N. Lipocalin-type prostaglandin d synthase in essential hypertension. Hypertension. 2002 Feb;39 (2 Pt 2):449-54
• Urade Y, Eguchi N, Sato N, Seiki K, Shiina Y, Oda H. Development and evaluation of a practical ELISA for human urinary lipocalin-type prostaglandin D synthase. Clin Chem. 2002 Sep;48 (9):1445-53

By research topic:

• Neural tissue damage markers
• Renal disease

By molecule:

• Prostaglandin D Synthase

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