PYY Rat ELISA

Research topic

Animal studies, Energy metabolism and body weight regulation

Storage/Shipping

Store all the components at 2–8°C.

Summary

PYY was isolated initially by Tatemoto et al. (1980) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level decreases after resection of the intestine, possiblly be due to the decrease in number of the endocrine cells secreting PYY.

Assay format

Competitive ELISA, Immobilized antibody

Sample requirements

25 µl/well

Applications

Plasma

Calibration Curve

Calibration range

0.14 to 100 ng/ml

Intra-assay

CV = 7.95–12.81 %

Inter-assay

CV = 11.95–13.61 %

References to summary

• Adrin, T.E., Smith, H.A, Calvert, S.A., Aynsley-Green, A.and Bloom, S.R.: Elevated plasma peptide YY in human neonates and infants.Pediatric Res., 20: 1225 – 1227 (1986).
• Adrian T.E., Ferri, G.L., Bacarese-Hamilton, A.J., Fuessl, H.S., Polak, J.M. and Bloom, S.R.: Human distribution and release of a putative new gut hormone, peptide YY.Gastroente­rology, 89: 1070–1077 (1995).
• El-Salhy, M., Grimelius, L ., Wilander, E ., Ryberg B., Terenius, L., Lundburg, J.M.and Tatemoto, K.: Immunocytochemical identification of polypeptide YY (PYY) cells in the human gastrointestinal tract.Histoche­mistry, 77: 15–23 (1983).
• Greeley, G.H.Jr., Hashimoto, T., Izukawa, M., Gomez, G., Jeng, J. Hill, F.L.C., Liuis, F.,and Tompson, J/C.: A comparison of intraduodenally and intracolonically administered nutrients on the release of peptide YY in the dog.Endrocrino­logy, 125: 1761–1765 (1989).
• Greeley, G.H.Jr., Hill, F. L. C., Spannagel, A.and Tompson, J.C.: Distribution of peptide YY in gastrointestinal tract of the rat dog, and monkey.Regul.Pep­t., 19, 365–372 (1987).
• Gomez, G., Zhang, T., Rajaraman, S., Thakore, K.N., Yanaihara, N., Townsendand C.M.Jr., Tompson, J.C. and Greeley, G.H.Jr.: Intestinal peptide YY : ontogeny of gene expression in rat bowel and trophic action on rat and mouse bowel.Am J.Physiol. 268, G71-G81 (1995).
• Larhammar, D.: Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide.Re­gul.Pept., 62, 1–11 (1996).