RBP4 Human ELISA

Summary

Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues (1). In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue (2). Yang et al. noted that adipocyte-specific deletion of Gluts led to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improved insulin resistance (3). This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. Thus, measurement of serum or plasma RBP4 is a useful means for understanding of metabolic disorders.

Research topic

Diabetology - Autoimmunity, Energy metabolism and body weight regulation

Assay format

Competitive ELISA, Immobilized antigen

Applications

Cell culture supernatant, Plasma, Serum, Urine

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.001 to 5 μg/ml

Limit of detection

1 ng/ml

References to summary

• Gottesman ME, Colantuoni V, Cosma MP, Freeman S, Gouras P, Piantedosi R, Vogel S, Salchow DJ, Blaner WS, Quadro L. Impaired retinal function and vitamin A availability in mice lacking retinol-binding protein. EMBO J. 1999 Sep 1;18 (17):4633-44
• Kahn BB, Quadro L, Kotani K, Zabolotny JM, Peroni OD, Preitner F, Mody N, Graham TE, Yang Q. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 2005 Jul 21;436 (7049):356-62
• Shepherd PR, Kahn BB. Glucose transporters and insulin action--implications for insulin resistance and diabetes mellitus. N Engl J Med. 1999 Jul 22;341 (4):248-57

By research topic:

• Diabetology - Autoimmunity
• Energy metabolism and body weight regulation

By molecule:

• Retinol Binding Protein 4