Resistin Rat ELISA

Research topic

Animal studies, Energy metabolism and body weight regulation

Features

• The total assay time is less than four hours.
• The kit measures total serum (homodimeric) resistin.
• Quality Controls are recombinant rat resistin based. No animal sera are used.

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Summary

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulace glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recent studies have shown that human resistin is expressed also in macrophages and may be a novel link between inflammation and insulin resistance.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

10 µl/well

Applications

Cell culture medium, Serum

Calibration Curve

Limit of detection

Analytical Limit of Detection is calculated from the real Resistin values in wells and is 0.05ng/ml

Limit of quantification

Assay Sensitivity takes the dilution of samples into consideration and is calculated according to the formula: Assay Sensitivity = Analytical Limit of Detection x sample dilution = 0.05ng/ml x 20 = 1ng/ml

Intra-assay (Within-Run, n=8)

CV = 5.1 %

Inter-assay (Run-to-Run, n=5)

CV = 7.1 %

Spiking Recovery

92.1 %

Dilution Linearity

112.7 %

Cross-Reactivity

Rat, Goat, Hamster

References to summary

• Steppan C.M., Lazar M.A.: The current biology of resistin. Journal of Internal Medicine. 255, 439–447, (2004)
• Gong H., Ni Y., Guo X., Fei L., Pan X., Guo M., Chen R.: Resistin promotes 3T3-L1 preadipocyte differentiation. Eur J Endocrinol. 150(6):885–92, (2004)
• Nogueiras R., Gallego R., Gualillo O., Caminos J.E., Garcia-Caballero T., Casanueva F.F., Dieguez C.: Resistin is expressed in different rat tissues and is regulated in a tissue- and gender-specific manner. FEBS Lett. 31;548(1–3):21–7, (2003)
• Satoh H., Nguyen M.T., Miles P.D., Imamura T., Usui I., Olefsky J.M.: Adenovirusmediated chronic “hyper-resistinemia” leads to in vivo insulin resistance in normal rats. J Clin Invest. 114(2):224–31, (2004)
• Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209–45215, (2003)
• Milan G., Granzotto M., Scarda A., Calcagno A., Pagano C., Federspil G., Vettor R.: Resistin and adiponectin expression in visceral fat of obese rats: effect of weight loss. Obes Res. 10(11):1095–103, (2002)
• Rajala M.W., Obici S., Scherer P.E., Rossetti L.: Adipose-derived resistin and gutderived resistin-like molecule-beta selectively impair insulin action on glucose production. J Clin Invest. 111(2):225–30, (2003)
• Steppan C.M., Lazar M.A.: Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18–23, (2002)
• Banerjee R.R., Lazar M.A.: Dimerization of resistin and resistin–like moleules is determined by a single cystein. The Journal of Biological Chemistry. 276, 25970–25973, (2001)
• Kim K.H. et al.: A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252–11256, (2001)
• Way J.M. et al.: Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological Chemistry. 276 (28), 25651–25653, (2001)
• Juan CC. at al.: Suppressed Gene Expression of Adipocyte Resistin in an Insulinresistant Rat Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research Communications. 289, 1328–1333, (2001)
• Steppan C.M. at al.: A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci. USA. 98 (2), 502–506, (2001)
• Steppan C.M. et al.: The Hormone Links Obesity to Diabetes. Nature. 409, 307–312, (2001)