s90K/Mac-2BP Human ELISA

Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Infection and inflammation, Oncology

Storage/Shipping

2–8°C

Summary

90K/Mac-2 BP (90 kDa) is a human serum glycoprotein, originally identified in the supernatant of human breast cancer cells.Serum levels of 90K/Mac-2 BP have been determined in patients with various forms of neoplasia and with some viral infections. In breast and colorectal cancer and in non-Hodgkin lymphoma, high levels of 90K/Mac-2 BP are correlated with a poor prognosis. The determination of 90K/Mac-2 BP in the serum can be used as a valuable parameter for monitoring the outcome of cancer and viral infections.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

100 µl (1:100 prediluted)

Applications

Cell culture supernatant, Serum

Calibration range

12.5 – 200 ng/ml

Limit of detection

0.92 ng/ml

Limit of quantification

92 ng/ml

Intra-assay

CV = 5.5%

Inter-assay

CV = 11.9%

Spiking Recovery

116%

Dilution Linearity

103.7%

References to summary

• Natoli C, Dianzani F, Mazzotta F, Balocchini E, Pierotti P, Antonelli G, Iacobelli S. 90K protein: a new predictor marker of disease progression in human immunodeficiency virus infection. J Acquir Immune Defic Syndr. 1993 Apr;6 (4):370-5
• Briggs NC, Natoli C, Tinari N, D'Egidio M, Goedert JJ, Iacobelli S. A 90-kDa protein serum marker for the prediction of progression to AIDS in a cohort of HIV-1+ homosexual men. AIDS Res Hum Retroviruses. 1993 Sep;9 (9):811-6
• Powell TJ, Schreck R, McCall M, Hui T, Rice A, App H, Azam M, Ullrich A, Shawver LK. A tumor-derived protein which provides T-cell costimulation through accessory cell activation. J Immunother Emphasis Tumor Im. 1995 May;17 (4):209-21
• Lotz MM, Andrews CW Jr, Korzelius CA, Lee EC, Steele GD Jr, Clarke A, Mercurio AM. Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma. Proc Natl Acad Sci U S A. 1993 Apr 15;90 (8):3466-70
• Iacobelli S, Arno E, D'Orazio A, Coletti G. Detection of antigens recognized by a novel monoclonal antibody in tissue and serum from patients with breast cancer. Cancer Res. 1986 Jun;46 (6):3005-10
• Natoli C, Garufi C, Tinari N, D'Egidio M, Lesti G, Gaspari LA, Visini R, Iacobelli S. Dynamic test with recombinant interferon-alpha-2b: effect on 90K and other tumour-associated antigens in cancer patients without evidence of disease. Br J Cancer. 1993 Mar;67 (3):564-7
• Artini M, Natoli C, Tinari N, Costanzo A, Marinelli R, Balsano C, Porcari P, Angelucci D, D'Egidio M, Levrero M, Iacobelli S. Elevated serum levels of 90K/MAC-2 BP predict unresponsiveness to alpha-interferon therapy in chronic HCV hepatitis patients. J Hepatol. 1996 Aug;25 (2):212-7
• Benedetti Panici P, Scambia G, Baiocchi G, Iacobelli S, Mancuso S. Predictive value of multiple tumor marker assays in second-look procedures for ovarian cancer. Gynecol Oncol. 1989 Dec;35 (3):286-9
• Iacobelli S, Sismondi P, Giai M, D'Egidio M, Tinari N, Amatetti C, Di Stefano P, Natoli C. Prognostic value of a novel circulating serum 90K antigen in breast cancer. Br J Cancer. 1994 Jan;69 (1):172-6
• Longo G, Natoli C, Rafanelli D, Tinari N, Morfini M, Rossi-Ferrini P, D'Ostilio N, Iacobelli S. Prognostic value of a novel circulating serum 90K antigen in HIV-infected haemophilia patients. Br J Haematol. 1993 Sep;85 (1):207-9
• Scambia G, Panici PB, Iacobelli S, Baiocchi G, Battaglia F, Perrone L, Sonsini C, Ferrandina G, Natoli C, Mancuso S. Recombinant alpha-2b-interferon enhances the circulating levels of a 90-kilodalton (K) tumor-associated antigen in patients with gynecologic and breast malignancies. Cancer. 1990 Mar 15;65 (6):1325-8
• Jallal B, Powell J, Zachwieja J, Brakebusch C, Germain L, Jacobs J, Iacobelli S, Ullrich A. Suppression of tumor growth in vivo by local and systemic 90K level increase. Cancer Res. 1995 Aug 1;55 (15):3223-7
• Ullrich A, Sures I, D'Egidio M, Jallal B, Powell TJ, Herbst R, Dreps A, Azam M, Rubinstein M, Natoli C, et al.. The secreted tumor-associated antigen 90K is a potent immune stimulator. J Biol Chem. 1994 Jul 15;269 (28):18401-7
• Natoli C, Iacobelli S, Ghinelli F. Unusually high level of a tumor-associated antigen in the serum of human immunodeficiency virus-seropositive individuals. J Infect Dis. 1991 Sep;164 (3):616-7