sCD28 Human ELISA

Summary

CD28 (44 kDa) is a disulphide bonded, homodimeric glycoprotein which is constitutively expressed on the surface of the majority of T-cells. Activating the CD28/B7 pathway could be useful in promoting recognition and elimination of tumors that had previously evaded the immune system.

Research topic

Cell surface proteins (sCD)

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.8 – 50 – ng/ml

Limit of detection

0.18 ng/ml

Intra-assay

CV = 5.9%

Inter-assay

CV = 7.2%

Spiking Recovery

117%

Dilution Linearity

105%

By research topic:

• Cell surface proteins (sCD)

By molecule:

• CD28

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