sCD30L Human ELISA

Summary

CD30 ligand is a 40 kDa Type II transmembrane glycoprotein. It interacts with its receptor, CD30, as a homotrimer. CD153 is a member of the TNF superfamily which is primarily expressed by activated T cells, B cells and monocytes. Its function in programmed cell death has been proven, and it has been shown that CD30L is involved in a variety of lymphoid malignancies like Hodgkin’s disease and anaplastic large cell lymphoma.

Research topic

Apoptosis, Cell surface proteins (sCD), Oncology

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

1.6 – 100 U/ml

Limit of detection

0.5 ng/ml

Intra-assay

CV = 8.7%

Inter-assay

CV = 8.7%

Spiking Recovery

82%

Dilution Linearity

105%

By research topic:

• Apoptosis
• Cell surface proteins (sCD)
• Oncology

By molecule:

• CD30L