sCD40 Human ELISA

Summary

CD40 (50 kDa) is a membrane-bound type I glycoprotein expressed by numerous cells, most notably B lymphocytes and monocytes and antigen presenting cells (APC) such as macrophages, dendritic cells and fibroblasts. CD40 belongs to the TNF-receptor family. Its critical role in T-cell-dependent humoral immune responses was demonstrated in patients with the hyper-IgM-Syndrome. The interaction of CD40 and its ligand, CD154 (CD40L) was found to play a crucial role in many aspects of immune response and the development and progress of various diseases.

Research topic

Apoptosis, Cell surface proteins (sCD), Oncology

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma, Plasma-EDTA, Plasma-Heparin

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 500 pg/ml

Limit of detection

6.92 pg/ml

Intra-assay

CV = 5.5%

Inter-assay

CV = 7.0%

Spiking Recovery

94%

Dilution Linearity

94%

By research topic:

• Apoptosis
• Cell surface proteins (sCD)
• Oncology

By molecule:

• CD40

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