sCD40L Human ELISA

Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Apoptosis, Autoimmunity, Cardiovascular disease, Cytokines and chemokines and related molecules, Growth hormone and factor-related products, Infection and inflammation, Transplantation

Storage/Shipping

Store kit reagents between 2°-8°C except controls. Store lyophilized controls at –20°C.

Summary

CD40 belongs to the TNF receptor superfamily. A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. This molecule as well as the other ligands of the family share the property of co-stimulation of T-cell proliferation and are all expressed by activated T-cells.Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T-helper cells. Thus the CD40/CD40L interaction plays a central role in the various phases of the B cell response to T-dependent antigens.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

20 µl

Applications

Cell culture supernatant, Plasma, Serum

Calibration Curve

Calibration range

0.16–10 ng/ml

Limit of detection

0.062 ng/ml

Limit of quantification

0.31 ng/ml

Intra-assay

CV = 4.0 %

Inter-assay

CV = 6.8 %

Spiking Recovery

91%

Dilution Linearity

105%

References to this product

• Cosman D. A family of ligands for the TNF receptor superfamily. Stem Cells. 1994 Sep;12 (5):440-55
• Rathmell JC, Townsend SE, Xu JC, Flavell RA, Goodnow CC. Expansion or elimination of B cells in vivo: dual roles for CD40- and Fas (CD95)-ligands modulated by the B cell antigen receptor. Cell. 1996 Oct 18;87 (2):319-29
• Bergman MC, Attrep JF, Grammer AC, Lipsky PE. Ligation of CD40 influences the function of human Ig-secreting B cell hybridomas both positively and negatively. J Immunol. 1996 May 1;156 (9):3118-32
• Tsubata T, Murakami M, Nisitani S, Honjo T. Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice. Philos Trans R Soc Lond B Biol. 1994 Aug 30;345 (1313):297-301
• Durie FH, Fava RA, Foy TM, Aruffo A, Ledbetter JA, Noelle RJ. Prevention of collagen-induced arthritis with an antibody to gp39, the ligand for CD40. Science. 1993 Sep 3;261 (5126):1328-30
• Rothstein TL, Wang JK, Panka DJ, Foote LC, Wang Z, Stanger B, Cui H, Ju ST, Marshak-Rothstein A. Protection against Fas-dependent Th1-mediated apoptosis by antigen receptor engagement in B cells. Nature. 1995 Mar 9;374 (6518):163-5
• Mazzei GJ, Edgerton MD, Losberger C, Lecoanet-Henchoz S, Graber P, Durandy A, Gauchat JF, Bernard A, Allet B, Bonnefoy JY. Recombinant soluble trimeric CD40 ligand is biologically active. J Biol Chem. 1995 Mar 31;270 (13):7025-8
• Lane P, Brocker T, Hubele S, Padovan E, Lanzavecchia A, McConnell F. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J Exp Med. 1993 Apr 1;177 (4):1209-13
• Brugnoni D, Rossi G, Tucci A, Cattaneo R, Airo P. Study of CD40 ligand expression in B-cell chronic lymphocytic leukemia. Haematologica. 1995 Sep-Oct;80 (5):440-2
• Holder MJ, Wang H, Milner AE, Casamayor M, Armitage R, Spriggs MK, Fanslow WC, MacLennan IC, Gregory CD, Gordon J, et al.. Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD40 ligand is independent of Bc1-2 induction. Eur J Immunol. 1993 Sep;23 (9):2368-71