sCD40L Human ELISA

Summary

CD40 belongs to the TNF receptor superfamily. A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. This molecule as well as the other ligands of the family share the property of co-stimulation of T-cell proliferation and are all expressed by activated T-cells.Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T-helper cells. Thus the CD40/CD40L interaction plays a central role in the various phases of the B cell response to T-dependent antigens.

Research topic

Apoptosis, Cardiovascular disease, Cell surface proteins (sCD), Cytokines and chemokines and related molecules, Transplantation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

20 µl

Storage/Shipping

Store kit reagents between 2°-8°C except controls. Store lyophilized controls at –20°C.

Calibration Curve

Calibration range

0.16–10 ng/ml

Limit of detection

0.06 ng/ml

Intra-assay

CV = 4.0 %

Inter-assay

CV = 6.8 %

Spiking Recovery

91%

Dilution Linearity

105%

References to this product

• Airo P, Cattaneo R, Tucci A, Rossi G, Brugnoni D. Study of CD40 ligand expression in B-cell chronic lymphocytic leukemia. Haematologica. 1995 Sep-Oct;80 (5):440-2
• Bonnefoy JY, Allet B, Bernard A, Gauchat JF, Durandy A, Graber P, Lecoanet-Henchoz S, Losberger C, Edgerton MD, Mazzei GJ. Recombinant soluble trimeric CD40 ligand is biologically active. J Biol Chem. 1995 Mar 31;270 (13):7025-8
• Cosman D. A family of ligands for the TNF receptor superfamily. Stem Cells. 1994 Sep;12 (5):440-55
• et al., Gordon J, Gregory CD, MacLennan IC, Fanslow WC, Spriggs MK, Armitage R, Casamayor M, Milner AE, Wang H, Holder MJ. Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD40 ligand is independent of Bc1-2 induction. Eur J Immunol. 1993 Sep;23 (9):2368-71
• Goodnow CC, Flavell RA, Xu JC, Townsend SE, Rathmell JC. Expansion or elimination of B cells in vivo: dual roles for CD40- and Fas (CD95)-ligands modulated by the B cell antigen receptor. Cell. 1996 Oct 18;87 (2):319-29
• Honjo T, Nisitani S, Murakami M, Tsubata T. Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice. Philos Trans R Soc Lond B Biol. 1994 Aug 30;345 (1313):297-301
• Lipsky PE, Grammer AC, Attrep JF, Bergman MC. Ligation of CD40 influences the function of human Ig-secreting B cell hybridomas both positively and negatively. J Immunol. 1996 May 1;156 (9):3118-32
• Marshak-Rothstein A, Ju ST, Cui H, Stanger B, Wang Z, Foote LC, Panka DJ, Wang JK, Rothstein TL. Protection against Fas-dependent Th1-mediated apoptosis by antigen receptor engagement in B cells. Nature. 1995 Mar 9;374 (6518):163-5
• McConnell F, Lanzavecchia A, Padovan E, Hubele S, Brocker T, Lane P. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J Exp Med. 1993 Apr 1;177 (4):1209-13
• Noelle RJ, Ledbetter JA, Aruffo A, Foy TM, Fava RA, Durie FH. Prevention of collagen-induced arthritis with an antibody to gp39, the ligand for CD40. Science. 1993 Sep 3;261 (5126):1328-30

By research topic:

• Apoptosis
• Cardiovascular disease
• Cell surface proteins (sCD)
• Cytokines and chemokines and related molecules
• Transplantation

By molecule:

• CD40 Ligand (CD40-L)

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