sCD40L Mouse ELISA

Research topic

Animal studies, Apoptosis, Autoimmunity, Cardiovascular disease, Cell surface proteins (sCD), Cytokines and chemokines and related molecules, Oncology, Transplantation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

Store kit reagents between 2° and 8°C. Immediately after use remaining reagents should be returned to cold storage (2° to 8°C).

Calibration Curve

Calibration range

0.31–20 ng/ml

Limit of detection

0.14 ng/ml

Intra-assay

CV = 6.5%

Inter-assay

CV = 11.1%

Spiking Recovery

85%

Dilution Linearity

96%

References to this product

• Cosman D. A family of ligands for the TNF receptor superfamily. Stem Cells. 1994 Sep;12 (5):440-55
• Rathmell JC, Townsend SE, Xu JC, Flavell RA, Goodnow CC. Expansion or elimination of B cells in vivo: dual roles for CD40- and Fas (CD95)-ligands modulated by the B cell antigen receptor. Cell. 1996 Oct 18;87 (2):319-29
• Tsubata T, Murakami M, Nisitani S, Honjo T. Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice. Philos Trans R Soc Lond B Biol. 1994 Aug 30;345 (1313):297-301
• Durie FH, Fava RA, Foy TM, Aruffo A, Ledbetter JA, Noelle RJ. Prevention of collagen-induced arthritis with an antibody to gp39, the ligand for CD40. Science. 1993 Sep 3;261 (5126):1328-30
• Rothstein TL, Wang JK, Panka DJ, Foote LC, Wang Z, Stanger B, Cui H, Ju ST, Marshak-Rothstein A. Protection against Fas-dependent Th1-mediated apoptosis by antigen receptor engagement in B cells. Nature. 1995 Mar 9;374 (6518):163-5
• Lane P, Brocker T, Hubele S, Padovan E, Lanzavecchia A, McConnell F. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J Exp Med. 1993 Apr 1;177 (4):1209-13

By research topic:

• Animal studies
• Apoptosis
• Autoimmunity
• Cardiovascular disease
• Cell surface proteins (sCD)
• Cytokines and chemokines and related molecules
• Oncology
• Transplantation

By molecule:

• CD40 Ligand (CD40-L)
• CD40L (Mouse)

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