sE-Selectin Human ELISA

Summary

E-selectin belongs to the selectin family of adhesion molecules. Together with LECAM-1 (L-selectin) and GMP-140 (P-selectin), E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells. E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of resting leukocytes to the endothelium. Determination of sE-selectin could provide more detailed insights into the pathological modifications during various diseases.

Research topic

Cell adhesion proteins , Immune Response, Infection and Inflammation, Transplantation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

20 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.8 – 50 ng/ml

Limit of detection

0.33 ng/ml

Intra-assay

CV = 5.4%

Inter-assay

CV = 6.0%

Spiking Recovery

86%

Dilution Linearity

95%

References to summary

• . A role for ELAM-1 in the pathogenesis of MOF during septic shock. J Surg Res. 1992 Aug;53 (2):136-44
• . Activation-independent binding of human memory T cells to adhesion molecule ELAM-1. Nature. 1991 Feb 28;349 (6312):799-802
• . Adhesion molecules are expressed in the human retina and choroid. Curr Eye Res. 1992;11 Suppl:153-9
• . Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo. J Immunol. 1991 Jan 15;146 (2):521-8
• . Endothelial leukocyte adhesion molecule-1 mediates antigen-induced acute airway inflammation and late-phase airway obstruction in monkeys. J Clin Invest. 1991 Oct;88 (4):1407-11
• . Expression of adhesion molecules in early allergic patch test reactions. Dermatology. 1992;185 (1):12-7
• . Expression of endothelial leukocyte adhesion molecule-1 in septic but not traumatic/hypovolemic shock in the baboon. Am J Pathol. 1991 Aug;139 (2):461-6
• . Expression of vascular adhesion molecules in inflammatory bowel disease. Gastroenterology. 1992 Sep;103 (3):840-7
• . L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263 (4 Pt 2):H1034-44
• . Lectin cell adhesion molecules (LEC-CAMs): a new family of cell adhesion proteins involved with inflammation. J Cell Biochem. 1991 Feb;45 (2):139-46
• . Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell. 1991 May 31;65 (5):859-73
• . Recruitment of neutrophils in the local endotoxin response: association with de novo endothelial expression of endothelial leukocyte adhesion molecule-1. Lab Invest. 1991 Feb;64 (2):295-9
• . Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats. J Clin Invest. 1991 Oct;88 (4):1396-406
• . Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatants of cytokine activated cultured endothelial cells. Biochem Biophys Res Commun. 1992 Sep 16;187 (2):584-9
• . The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide. J Cell Biol. 1992 May;117 (4):895-902
• Brasch J, Sterry W. Expression of adhesion molecules in early allergic patch test reactions. Dermatology. 1992;185 (1):12-7
• Duguid IG, Boyd AW, Mandel TE. Adhesion molecules are expressed in the human retina and choroid. Curr Eye Res. 1992;11 Suppl:153-9
• Engelberts I, Samyo SK, Leeuwenberg JF, van der Linden CJ, Buurman WA. A role for ELAM-1 in the pathogenesis of MOF during septic shock. J Surg Res. 1992 Aug;53 (2):136-44
• Foxall C, Watson SR, Dowbenko D, Fennie C, Lasky LA, Kiso M, Hasegawa A, Asa D, Brandley BK. The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide. J Cell Biol. 1992 May;117 (4):895-902
• Gundel RH, Wegner CD, Torcellini CA, Clarke CC, Haynes N, Rothlein R, Smith CW, Letts LG. Endothelial leukocyte adhesion molecule-1 mediates antigen-induced acute airway inflammation and late-phase airway obstruction in monkeys. J Clin Invest. 1991 Oct;88 (4):1407-11
• Koizumi M, King N, Lobb R, Benjamin C, Podolsky DK. Expression of vascular adhesion molecules in inflammatory bowel disease. Gastroenterology. 1992 Sep;103 (3):840-7
• Kyan-Aung U, Haskard DO, Poston RN, Thornhill MH, Lee TH. Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo. J Immunol. 1991 Jan 15;146 (2):521-8
• Lasky LA. Lectin cell adhesion molecules (LEC-CAMs): a new family of cell adhesion proteins involved with inflammation. J Cell Biochem. 1991 Feb;45 (2):139-46
• Lawrence MB, Springer TA. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell. 1991 May 31;65 (5):859-73
• Mulligan MS, Varani J, Dame MK, Lane CL, Smith CW, Anderson DC, Ward PA. Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats. J Clin Invest. 1991 Oct;88 (4):1396-406
• Munro JM, Pober JS, Cotran RS. Recruitment of neutrophils in the local endotoxin response: association with de novo endothelial expression of endothelial leukocyte adhesion molecule-1. Lab Invest. 1991 Feb;64 (2):295-9
• Pigott R, Dillon LP, Hemingway IH, Gearing AJ. Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatants of cytokine activated cultured endothelial cells. Biochem Biophys Res Commun. 1992 Sep 16;187 (2):584-9
• Redl H, Dinges HP, Buurman WA, van der Linden CJ, Pober JS, Cotran RS, Schlag G. Expression of endothelial leukocyte adhesion molecule-1 in septic but not traumatic/hypovolemic shock in the baboon. Am J Pathol. 1991 Aug;139 (2):461-6
• Shimizu Y, Shaw S, Graber N, Gopal TV, Horgan KJ, Van Seventer GA, Newman W. Activation-independent binding of human memory T cells to adhesion molecule ELAM-1. Nature. 1991 Feb 28;349 (6312):799-802
• Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE. L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263 (4 Pt 2):H1034-44

By research topic:

• Cell adhesion proteins
• Immune Response, Infection and Inflammation
• Transplantation

By molecule:

• E-Selectin
• Selectin

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