Secretagogin Human ELISA

Features

• It is intended for research use only
• The total assay time is less than 3.5 hours
• The kit measures human secretagogin in serum and plasma (EDTA)
• Quality Controls are human serum based. No animal sera are used
• Standard is recombinant protein based
• Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Diabetology - Autoimmunity, Diabetology - Other Relevant Products, Neural tissue damage markers, Oncology

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma-EDTA, Serum

Sample requirements

50 µl/well

Calibration Curve

Calibration range

62.5 to 2000 pg/ml

Limit of detection

11 pg/ml

Intra-assay

CV = 6,02%

Inter-assay

CV = 6,48%

Spiking Recovery

91,60%

Dilution Linearity

106%

References to this product

• Fedora M, Zurek J. The usefulness of S100B, NSE, GFAP, NF-H, secretagogin and Hsp70 as a predictive biomarker of outcome in children with traumatic brain injury. Acta Neurochir (Wien). 2011 Oct 7;

References to summary

• Berggard T, Wagner L, James P, Lindqvist A, Linse S, Rogstam A. Binding of calcium ions and SNAP-25 to the hexa EF-hand protein secretagogin. Biochem J. 2007 Jan 1;401 (1):353-63
• Huang Q, Ren G, Xu E, Xing X, Lu B, Lai M. Secretagogin, a novel neuroendocrine marker, has a distinct expression pattern from chromogranin A. Virchows Arch. 2006 Oct;449 (4):402-9
• Preusser M, Budka H, Rossler K, Wagner L, Pipp I. Secretagogin expression in tumours of the human brain and its coverings. APMIS. 2007 Apr;115 (4):319-26
• Torring N, Orntoft TF, Birkenkamp-Demtroder K, Borre M, Ottosen P, Stattin P, Bergh A, Wagner L, Adolf K. Secretagogin is a new neuroendocrine marker in the human prostate. Prostate. 2007 Apr 1;67 (5):472-84
• Wagner L, Luger A, Majdic O, Ilhan A, Koc-Saral F, Nabokikh A, Daneva T, Vila G, Gartner W. New functional aspects of the neuroendocrine marker secretagogin based on the characterization of its rat homolog. Am J Physiol Endocrinol Metab. 2007 Jul;293 (1):E347-54
• Wagner L, Pasternack MS, Waldhausl W, Kaserer K, Groeger M, Nowotny P, Gartner W, Oliyarnyk O. Cloning and expression of secretagogin, a novel neuroendocrine- and pancreatic islet of Langerhans-specific Ca2+-binding protein. J Biol Chem. 2000 Aug 11;275 (32):24740-51
• Wagner L, Waldhausl W, Domanovits H, Leutmetzer F, Lang W, Gartner W. Cerebral expression and serum detectability of secretagogin, a recently cloned EF-hand Ca(2+)-binding protein. Cereb Cortex. 2001 Dec;11 (12):1161-9
• Wagner L, Weissel M, Base W, Wolf F, Prayer D, Domanovits H, Leutmezer F, Sodeck G, Mineva I, Zierhut B, Gartner W. Brain natriuretic peptide correlates with the extent of atrial fibrillation-associated silent brain lesions. Clin Biochem. 2008 Dec;41 (18):1434-9

By research topic:

• Diabetology - Autoimmunity
• Diabetology - Other Relevant Products
• Neural tissue damage markers
• Oncology

By molecule:

• Secretagogin

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