sFas Ligand Human ELISA

Summary

Fas is a type I membrane protein that belongs to the TNF/nerve growth factor receptor family. Fas mediates apoptosis, the programmed cell death, when it is cross-linked with specific binding partners. FasL is predominantly expressed on activated T-cells and NK cells; thus FasL-mediated cell death is involved in the T or NK cell-mediated cytotoxicity, some pathologic tissue damages, and the regulation of lymphocyte homeostasis. Elevated expression levels of FasL have been measured in various proliferative disorders and cancers.

Research topic

Apoptosis

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.16 – 10 ng/

Limit of detection

0.07 ng/ml

Intra-assay

CV = 6.1%

Inter-assay

CV = 7.0%

Spiking Recovery

85%

Dilution Linearity

106 %

By research topic:

• Apoptosis

By molecule:

• Fas Ligand