sICAM-1 Human ELISA

Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Cell adhesion proteins

Storage/Shipping

Store kit reagents between 2° and 8°C except control. Store lyophilized control at –20°C.

Summary

ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFNγ within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations. ICAM-1 upregulation in allergic airway inflammation is responsible for the recruitment of activated leukocytes and the pathogenesis of allergic rhinitis.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

10 µl

Applications

Amniotic fluid, bile, Cell culture supernatant, Plasma-EDTA, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

6.25–100 ng/ml

Limit of detection

2.2 ng/ml

Limit of quantification

22 ng/ml

Intra-assay

CV = 4.1 %

Inter-assay

CV = 7.7 %

Spiking Recovery

99%.

Dilution Linearity

93%

References to summary

• Rothlein R, Mainolfi EA, Czajkowski M, Marlin SD. A form of circulating ICAM-1 in human serum. J Immunol. 1991 Dec 1;147 (11):3788-93
• Rothlein R, Dustin ML, Marlin SD, Springer TA. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J Immunol. 1986 Aug 15;137 (4):1270-4
• Arocker-Mettinger E, Steurer-Georgiew L, Steurer M, Huber-Spitzy V, Hoelzl E, Grabner G, Kuchar A. Circulating ICAM-1 levels in serum of uveitis patients. Curr Eye Res. 1992;11 Suppl:161-6
• Altomonte M, Colizzi F, Esposito G, Maio M. Circulating intercellular adhesion molecule 1 as a marker of disease progression in cutaneous melanoma. N Engl J Med. 1992 Sep 24;327 (13):959
• Salafia CM, DeVore GR, Mainolfi E, Kelly J, Pezzullo JC, Rothlein R. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am J Obstet Gynecol. 1993 Oct;169 (4):830-4
• Becker JC, Dummer R, Schwinn A, Hartmann AA, Burg G. Circulating intercellular adhesion molecule-1 in melanoma patients: induction by interleukin-2 therapy. J Immunother (1991). 1992 Aug;12 (2):147-50
• Adams DH, Mainolfi E, Elias E, Neuberger JM, Rothlein R. Detection of circulating intercellular adhesion molecule-1 after liver transplantation--evidence of local release within the liver during graft rejection. Transplantation. 1993 Jan;55 (1):83-7
• Most J, Zangerle R, Herold M, Fuchs D, Wachter H, Fritsch P, Dierich MP. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J Acquir Immune Defic Syndr. 1993 Mar;6 (3):221-6
• Lal RB, Mainolfi E, Rothlein R. Elevated levels of cICAM-1 in patients with human T-cell leukemia virus type I associated myelopathy and adult T-cell leukemia. Blood. 1992 Nov 1;80 (9):2434-5
• Lampeter ER, Kishimoto TK, Rothlein R, Mainolfi EA, Bertrams J, Kolb H, Martin S. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes. 1992 Dec;41 (12):1668-71
• Potocnik AJ, Kinne R, Menninger H, Zacher J, Emmrich F, Kroczek RA. Expression of activation antigens on T cells in rheumatoid arthritis patients. Scand J Immunol. 1990 Feb;31 (2):213-24
• Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol. 1986 Jul 1;137 (1):245-54
• Rothlein R, Czajkowski M, O'Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J Immunol. 1988 Sep 1;141 (5):1665-9
• Adams DH, Hubscher SG, Shaw J, Rothlein R, Neuberger JM. Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet. 1989 Nov 11;2 (8672):1122-5
• Wegner CD, Gundel RH, Reilly P, Haynes N, Letts LG, Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science. 1990 Jan 26;247 (4941):456-9
• Lisby S, Ralfkiaer E, Rothlein R, Vejlsgaard GL. Intercellular adhesion molecule-I (ICAM-I) expression correlated to inflammation. Br J Dermatol. 1989 Apr;120 (4):479-84
• Vejlsgaard GL, Ralfkiaer E, Avnstorp C, Czajkowski M, Marlin SD, Rothlein R. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphomas. J Am Acad Dermatol. 1989 May;20 (5 Pt 1):782-90
• Pober JS, Gimbrone MA Jr, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J Immunol. 1986 Sep 15;137 (6):1893-6
• Marlin SD, Springer TA. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell. 1987 Dec 4;51 (5):813-9
• Harning R, Mainolfi E, Bystryn JC, Henn M, Merluzzi VJ, Rothlein R. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991 Sep 15;51 (18):5003-5