sICAM-2 Human ELISA

Summary

ICAM-2 (60 kDa) is a surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. It is distributed on hematopoietic cells. ICAM-2 dominates over ICAM-1 in resting endothelial cells and seems to be involved in the recirculation of LFA-1 positive lymphocytes ( e.g. facilitating T-memory cell recirculation). As resting T-cells express little or no ICAM-1, ICAM-2 may also be important in initial T-cell adhesion with antigen-presenting cells that bear LFA-1.

Research topic

Cell adhesion proteins

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma, Serum, Urine, Whole blood

Sample requirements

100 µl (1:20 prediluted)

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.75 – 24 U/ml

Limit of detection

0.30 U/ml

Intra-assay

CV = 6.7%

Inter-assay

CV = 7.0%

By research topic:

• Cell adhesion proteins

By molecule:

• ICAM-2