sICAM-3 Human ELISA

Summary

ICAM-3 is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1. ICAM-3 is a heavily glycosylated protein of 124 kDa with a polypeptide core of 57 kDa. ICAM-3 is absent in endothelia. It is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. ICAM-3 is a very interesting molecule involved in the initial immune response, thus suggesting an important role as a disease marker for a number of different indications and pathological situations.

Research topic

Cell adhesion proteins

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma, Serum, Urine

Sample requirements

20 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.8 – 50 ng/ml

Limit of detection

0.38 ng/ml

Intra-assay

CV = 2.5%

Inter-assay

CV = 2.0%

Spiking Recovery

106 %

Dilution Linearity

96 %

By research topic:

• Cell adhesion proteins

By molecule:

• ICAM-3