sP-Selectin Human ELISA

Summary

P-selectin belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium. P-selectin-mediated adhesive interactions operate in conjunction with cell-cell interactions directed by related molecules and are likely to be important in both hemostatic and inflammatory processes. The physiological role of P-selectin may be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. Soluble P-selectin is a potentially important molecule that may provide more detailed insight into pathological situations.

Research topic

Cell adhesion proteins

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Plasma, Serum

Sample requirements

10 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

2.19 – 140 ng/ml

Limit of detection

1.6 ng/ml

Intra-assay

CV = 2.4%

Inter-assay

CV = 5.2%

Spiking Recovery

94%

Dilution Linearity

108%

By research topic:

• Cell adhesion proteins

By molecule:

• P-Selectin
• Selectin