sPECAM-1 Human ELISA

Summary

PECAM-1 is a newly characterized adhesion molecule that belongs to the immunoglobulin superfamily. It is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. PECAM-1 is a sensitive and specific marker for detection of melanoma-associated angiogenesis. It may be a useful marker for early detection of tumor metastasis.

Research topic

Cell adhesion proteins

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum, Urine

Sample requirements

10 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.48 – 30 ng/ml

Limit of detection

0.06 ng/ml

Intra-assay

CV = 1.7%

Inter-assay

CV = 7.4%

Spiking Recovery

108 %

Dilution Linearity

91.4 %

By research topic:

• Cell adhesion proteins

By molecule:

• PECAM-1

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