sPSGL-1 Human ELISA

Summary

PSGL-1 a transmembrane protein which forms homodimers via disulfide bridges of two 120 kDa chains. It is expressed on cells of myeloid, lymphoid and dendritic lineage.

Research topic

Cell adhesion proteins

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

100 µl (1:20 prediluted)

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

1.6 – 50 U/ml

Limit of detection

0.99 U/ml

Intra-assay

CV = 3.2%

Inter-assay

CV = 6.6%

Spiking Recovery

94%

Dilution Linearity

95.3 %

By research topic:

• Cell adhesion proteins

By molecule:

• PSGL-1