sTfR Human ELISA (soluble Transferrin Receptor)

Summary

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Features

• European Union: for in vitro diagnostic use

Rest of the world: for research use only!

• The total assay time is less than 3 hours
• The kit measures total soluble transferrin receptor in serum and plasma (EDTA, citrate, heparin)
• Assay format is 96 wells
• Quality Controls are human serum based. No animal sera are used
• Standard is natural human blood isolated sTfR based
• Components of the kit are provided ready to use or concentrated

Research topic

Iron metabolism

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

10 µl/well

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

0.05 – 2 µg/ml

Limit of detection

2 ng/ml

Intra-assay (Within-Run, n=8)

CV = 6.8 %

Inter-assay (Run-to-Run, n=4)

CV = 6.3 %

Spiking Recovery

100.2%

Dilution Linearity

94 %

Cross-Reactivity

References to this product

• Fernandez-Real JM, Izquierdo M, Moreno-Navarrete JM, Gorostiaga E, Ortega F, Martinez C, Idoate F, Ricart W, Ibanez J. Circulating soluble transferrin receptor concentration decreases after exercise-induced improvement of insulin sensitivity in obese individuals. Int J Obes (Lond). 2009 Jul;33 (7):768-74
• Fernandez-Real JM, Moreno JM, Lopez-Bermejo A, Chico B, Vendrell J, Ricart W. Circulating soluble transferrin receptor according to glucose tolerance status and insulin sensitivity. Diabetes Care . Mar;30(3):604-8 (2007)
• Khambalia A, O'Connor DL, Zlotkin S. Periconceptional iron and folate status is inadequate among married, nulliparous women in rural Bangladesh. J Nutr. 2009 Jun;139 (6):1179-84
• Mojiminiyi OA, Marouf R, Abdella NA. Body iron stores in relation to the metabolic syndrome, glycemic control and complications in female patients with type 2 diabetes. Nutr Metab Cardiovasc Dis. 2008 Oct;18 (8):559-66
• Shinzato T, Abe K, Furusu A, Harada T, Shinzato K, Miyazaki M, Kohno S. Serum pro-hepcidin level and iron homeostasis in Japanese dialysis patients with erythropoietin (EPO)-resistant anemia. Med Sci Monit. 2008 Sep;14 (9):CR431-7

References to summary

• Raya G. et al.: Soluble transferrin receptor (sTfR): biological variations and reference limits. Clinical Chemistry and Laboratory Medicine, 39, 1162–1168 (2001)
• Cotton F. et al.: Measurement of soluble transferrin receptor by immunoturbidimety and immunonefelometry. Clinical Biochemistry, 33, 263–267 (2000)
• Cook J. D.: The measurement of serum transferrin receptor. The American Journal of the Medical Sciences 318, 269–276 (1999)
• Olivares M. et al.: Usefulness of serum transferrin receptor and serum ferritin in diagnosis of iron deficiency in infancy. American Journal of Clinical Nutrition 72, 1191–1195 (2000)
• Suominen P. et al.: Single values of serum transferrin receptor and transferrin receptor ferritin index can be used to detect true and functional iron deficiency in rheumatoid arthritis patients with anemia. Arthritis & Rheumatism 43, 1016–1020 (2000)
• De Block C. E. M. et al.: Soluble transferrin receptor level. A new marker for iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1diabetes. Diabetes Care 23, 1384–1388 (2000)
• Kolbe-Busch S. et al. Determination of the soluble transferrin receptor in Serum: Evaluation of two enzyme immunoassays and a particle-enhanced immunonephelometric assay. Clinical Laborators 45, 295–304 (1999)
• Hikawa A. et al.: Soluble transferrin receptor-transferrin complex in serum: measurement by latex agglutination nephelometric asssay. Clinica Chimica Acta 254, 159–172 (1996)
• Flowers C. H. et al.: The clinical measurement of serum transferrin receptor. Journal of Laboratory and Clinical Medicine 114, 368–377 (1989)

By research topic:

• Iron metabolism

By molecule:

• Transferrin receptor

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