sTNF-R (60 kDa) Human ELISA

Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Cytokines and chemokines and related molecules

Storage/Shipping

Store kit reagents between 2–8°C except controls. Store lyophilized controls at –20°C.

Summary

Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNFα, also called cachectin, and TNFβ, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kDa) in various neoplastic and inflammatory diseases.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

10 µl

Applications

Cell culture supernatant, Plasma, Serum, Urine

Calibration Curve

Calibration range

0.08–5 ng/ml

Limit of detection

53 pg/ml

Limit of quantification

530 pg/ml

Intra-assay

CV = 1.9 %

Inter-assay

CV = 8.6 %

Spiking Recovery

93%

Dilution Linearity

103%

References to summary

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