sTNF-R (80kDa) Human ELISA

Summary

Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNFα, also called cachectin, and TNFβ, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum, Urine

Sample requirements

10 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.16 – 10 ng/ml

Limit of detection

0.09 ng/ml

Intra-assay

CV = 1.4%

Inter-assay

CV = 2.0%

Spiking Recovery

94 %

Dilution Linearity

93.5 %

References to summary

• . A high molecular weight component of the human tumor necrosis factor receptor is associated with cytotoxicity. Proc Natl Acad Sci U S A. 1987 May;84 (10):3293-7
• . A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
• . A receptor for tumor necrosis factor defines an unusual family of cellular and viral proteins. Science. 1990 May 25;248 (4958):1019-23
• . A tumor necrosis factor binding protein is present in human biological fluids. Eur J Haematol. 1988 Nov;41 (5):414-9
• . An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
• . Binding of human tumor necrosis factor to high affinity receptors on HeLa and lymphoblastoid cells sensitive to growth inhibition. J Biol Chem. 1985 Nov 5;260 (25):13395-7
• . Blocking factors (soluble membrane receptors) for tumor necrosis factor and lymphotoxin detected in ascites and released in short-term cultures obtained from ascites and solid tumors in women with gynecologic malignancy. Lymphokine Cytokine Res. 1992 Dec;11 (6):347-53
• . Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
• . Cellular receptor for 125I-labeled tumor necrosis factor: specific binding, affinity labeling, and relationship to sensitivity. Proc Natl Acad Sci U S A. 1985 Sep;82 (17):5756-60
• . Characterization of receptors for human tumour necrosis factor and their regulation by gamma-interferon. Nature. 1985 Dec 19-1986 Jan 1;318 (6047):665-7
• . Circulating soluble tumor necrosis factor receptors, interleukin-2 receptors, tumor necrosis factor alpha, and interleukin-6 levels in rheumatoid arthritis. Longitudinal evaluation during methotrexate and azathioprine therapy. Arthritis Rheum. 1993 Aug;36 (8):1070-9
• . Correlation between serum levels of soluble tumor necrosis factor receptor and disease activity in systemic lupus erythematosus. Arthritis Rheum. 1993 Aug;36 (8):1111-20
• . Elevated serum levels of soluble tumour necrosis factor receptors (sTNF-R) in patients with HIV infection. Clin Exp Immunol. 1992 Sep;89 (3):351-5
• . Evaluation of soluble tumor necrosis factor (TNF) receptors and TNF receptor antibodies in patients with systemic lupus erythematodes, progressive systemic sclerosis, and mixed connective tissue disease. J Clin Immunol. 1993 Sep;13 (5):321-8
• . Evidence of participation of the soluble tumor necrosis factor receptor I in the host response to intrauterine infection in preterm labor. Am J Reprod Immunol. 1993 Sep-Oct;30 (2-3):184-93
• . High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors. J Exp Med. 1985 Sep 1;162 (3):1099-104
• . High concentrations of soluble tumor necrosis factor receptors in ascites. Hepatology. 1992 Sep;16 (3):749-55
• . Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med. 1990 Oct 1;172 (4):1019-23
• . Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc Natl Acad Sci U S A. 1990 Apr;87 (8):3127-31
• . Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
• . Increased levels of soluble tumor necrosis factor-alpha receptors in serum from pregnant women and in serum and urine samples from newborns. Pediatr Res. 1993 Jan;33 (1):82-7
• . Infusion of tumor necrosis factor (TNF) causes an increase in circulating TNF-binding protein in humans. Cytokine. 1990 Nov;2 (6):402-6
• . Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
• . Molecular cloning and expression of a receptor for human tumor necrosis factor. Cell. 1990 Apr 20;61 (2):361-70
• . Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
• . Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor. Cell. 1990 Apr 20;61 (2):351-9
• . Purification of cachectin, a lipoprotein lipase-suppressing hormone secreted by endotoxin-induced RAW 264.7 cells. J Exp Med. 1985 May 1;161 (5):984-95
• . Quantification and characterization of high-affinity membrane receptors for tumor necrosis factor on human leukemic cell lines. Int J Cancer. 1986 Jul 15;38 (1):127-33
• . Soluble receptors for tumour necrosis factor: a putative marker of disease progression in HIV infection. AIDS. 1993 Jan;7 (1):33-6
• . Soluble tumor necrosis factor receptors correlate with parasitemia and disease severity in human malaria. J Infect Dis. 1992 Oct;166 (4):930-4
• . The divergent receptors for TNF. Trends Biochem Sci. 1990 Oct;15 (10):366-8
• . The significance of serum levels of soluble 60kDa receptors for tumor necrosis factor in patients with Hodgkin's disease. Leukemia. 1993 Sep;7 (9):1339-43
• . Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
• . Tumor necrosis factor and its receptors in human ovarian cancer. Potential role in disease progression. J Clin Invest. 1993 May;91 (5):2194-206
• . Tumor necrosis factor up-regulates gamma-interferon binding in a human carcinoma cell line. J Biol Chem. 1990 Jun 25;265 (18):10466-72
• . Tumor necrosis factor: receptors on hematopoietic cells. Blood. 1987 Dec;70 (6):1730-4
• . Tumor necrosis factor: specific binding and internalization in sensitive and resistant cells. Proc Natl Acad Sci U S A. 1985 Nov;82 (22):7626-30
• . Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha). J Biol Chem. 1989 Sep 5;264 (25):14927-34
• Aderka D, Wysenbeek A, Engelmann H, Cope AP, Brennan F, Molad Y, Hornik V, Levo Y, Maini RN, Feldmann M, et al. Correlation between serum levels of soluble tumor necrosis factor receptor and disease activity in systemic lupus erythematosus. Arthritis Rheum. 1993 Aug;36 (8):1111-20
• Adolf GR, Apfler I. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
• Aggarwal BB, Eessalu TE, Hass PE. Characterization of receptors for human tumour necrosis factor and their regulation by gamma-interferon. Nature. 1985 Dec 19-1986 Jan 1;318 (6047):665-7
• Andus T, Gross V, Holstege A, Ott M, Weber M, David M, Gallati H, Gerok W, Scholmerich J. High concentrations of soluble tumor necrosis factor receptors in ascites. Hepatology. 1992 Sep;16 (3):749-55
• Austgulen R, Liabakk NB, Lien E, Espevik T. Increased levels of soluble tumor necrosis factor-alpha receptors in serum from pregnant women and in serum and urine samples from newborns. Pediatr Res. 1993 Jan;33 (1):82-7
• Baglioni C, McCandless S, Tavernier J, Fiers W. Binding of human tumor necrosis factor to high affinity receptors on HeLa and lymphoblastoid cells sensitive to growth inhibition. J Biol Chem. 1985 Nov 5;260 (25):13395-7
• Barrera P, Boerbooms AM, Janssen EM, Sauerwein RW, Gallati H, Mulder J, de Boo T, Demacker PN, van de Putte LB, van der Meer JW. Circulating soluble tumor necrosis factor receptors, interleukin-2 receptors, tumor necrosis factor alpha, and interleukin-6 levels in rheumatoid arthritis. Longitudinal evaluation during methotrexate and azathioprine therapy. Arthritis Rheum. 1993 Aug;36 (8):1070-9
• Baumann P, Romero R, Berry S, Gomez R, McFarlin B, Araneda H, Cotton DB, Fidel P. Evidence of participation of the soluble tumor necrosis factor receptor I in the host response to intrauterine infection in preterm labor. Am J Reprod Immunol. 1993 Sep-Oct;30 (2-3):184-93
• Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
• Beutler B, Mahoney J, Le Trang N, Pekala P, Cerami A. Purification of cachectin, a lipoprotein lipase-suppressing hormone secreted by endotoxin-induced RAW 264.7 cells. J Exp Med. 1985 May 1;161 (5):984-95
• Brockhaus M, Schoenfeld HJ, Schlaeger EJ, Hunziker W, Lesslauer W, Loetscher H. Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc Natl Acad Sci U S A. 1990 Apr;87 (8):3127-31
• Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
• Creasey AA, Yamamoto R, Vitt CR. A high molecular weight component of the human tumor necrosis factor receptor is associated with cytotoxicity. Proc Natl Acad Sci U S A. 1987 May;84 (10):3293-7
• Godfried MH, van der Poll T, Jansen J, Romijin JA, Schattenkerk JK, Endert E, van Deventer SJ, Sauerwein HP. Soluble receptors for tumour necrosis factor: a putative marker of disease progression in HIV infection. AIDS. 1993 Jan;7 (1):33-6
• Grosen EA, Yamamoto RS, Ioli G, Ininns EK, Gatanaga M, Gatanaga T, DiSaia PJ, Berman M, Manetta A, Granger GA. Blocking factors (soluble membrane receptors) for tumor necrosis factor and lymphotoxin detected in ascites and released in short-term cultures obtained from ascites and solid tumors in women with gynecologic malignancy. Lymphokine Cytokine Res. 1992 Dec;11 (6):347-53
• Gruss HJ, Dolken G, Brach MA, Mertelsmann R, Herrmann F. The significance of serum levels of soluble 60kDa receptors for tumor necrosis factor in patients with Hodgkin's disease. Leukemia. 1993 Sep;7 (9):1339-43
• Heilig B, Fiehn C, Brockhaus M, Gallati H, Pezzutto A, Hunstein W. Evaluation of soluble tumor necrosis factor (TNF) receptors and TNF receptor antibodies in patients with systemic lupus erythematodes, progressive systemic sclerosis, and mixed connective tissue disease. J Clin Immunol. 1993 Sep;13 (5):321-8
• Himmler A, Maurer-Fogy I, Kronke M, Scheurich P, Pfizenmaier K, Lantz M, Olsson I, Hauptmann R, Stratowa C, Adolf GR. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
• Hohmann HP, Remy R, Brockhaus M, van Loon AP. Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha). J Biol Chem. 1989 Sep 5;264 (25):14927-34
• Kalinkovich A, Engelmann H, Harpaz N, Burstein R, Barak V, Kalickman I, Wallach D, Bentwich Z. Elevated serum levels of soluble tumour necrosis factor receptors (sTNF-R) in patients with HIV infection. Clin Exp Immunol. 1992 Sep;89 (3):351-5
• Kern P, Hemmer CJ, Gallati H, Neifer S, Kremsner P, Dietrich M, Porzsolt F. Soluble tumor necrosis factor receptors correlate with parasitemia and disease severity in human malaria. J Infect Dis. 1992 Oct;166 (4):930-4
• Kull FC Jr, Jacobs S, Cuatrecasas P. Cellular receptor for 125I-labeled tumor necrosis factor: specific binding, affinity labeling, and relationship to sensitivity. Proc Natl Acad Sci U S A. 1985 Sep;82 (17):5756-60
• Lantz M, Malik S, Slevin ML, Olsson I. Infusion of tumor necrosis factor (TNF) causes an increase in circulating TNF-binding protein in humans. Cytokine. 1990 Nov;2 (6):402-6
• Loetscher H, Pan YC, Lahm HW, Gentz R, Brockhaus M, Tabuchi H, Lesslauer W. Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor. Cell. 1990 Apr 20;61 (2):351-9
• Munker R, DiPersio J, Koeffler HP. Tumor necrosis factor: receptors on hematopoietic cells. Blood. 1987 Dec;70 (6):1730-4
• Naylor MS, Stamp GW, Foulkes WD, Eccles D, Balkwill FR. Tumor necrosis factor and its receptors in human ovarian cancer. Potential role in disease progression. J Clin Invest. 1993 May;91 (5):2194-206
• Old LJ. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
• Paul NL, Ruddle NH. Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
• Peetre C, Thysell H, Grubb A, Olsson I. A tumor necrosis factor binding protein is present in human biological fluids. Eur J Haematol. 1988 Nov;41 (5):414-9
• Raitano AB, Korc M. Tumor necrosis factor up-regulates gamma-interferon binding in a human carcinoma cell line. J Biol Chem. 1990 Jun 25;265 (18):10466-72
• Rubin BY, Anderson SL, Sullivan SA, Williamson BD, Carswell EA, Old LJ. High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors. J Exp Med. 1985 Sep 1;162 (3):1099-104
• Schall TJ, Lewis M, Koller KJ, Lee A, Rice GC, Wong GH, Gatanaga T, Granger GA, Lentz R, Raab H, et al. Molecular cloning and expression of a receptor for human tumor necrosis factor. Cell. 1990 Apr 20;61 (2):361-70
• Scheurich P, Thoma B, Ucer U, Pfizenmaier K. Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
• Scheurich P, Ucer U, Kronke M, Pfizenmaier K. Quantification and characterization of high-affinity membrane receptors for tumor necrosis factor on human leukemic cell lines. Int J Cancer. 1986 Jul 15;38 (1):127-33
• Smith CA, Davis T, Anderson D, Solam L, Beckmann MP, Jerzy R, Dower SK, Cosman D, Goodwin RG. A receptor for tumor necrosis factor defines an unusual family of cellular and viral proteins. Science. 1990 May 25;248 (4958):1019-23
• Sprang SR. The divergent receptors for TNF. Trends Biochem Sci. 1990 Oct;15 (10):366-8
• Thoma B, Grell M, Pfizenmaier K, Scheurich P. Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med. 1990 Oct 1;172 (4):1019-23
• Tsujimoto M, Yip YK, Vilcek J. Tumor necrosis factor: specific binding and internalization in sensitive and resistant cells. Proc Natl Acad Sci U S A. 1985 Nov;82 (22):7626-30

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• TNF-R (80 kDa)

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