Surfactant Protein D Human ELISA

Summary

SP-D is a homotrimeric protein consisting of three 43kDa units, that are bonded at their N-termini. Most preparations of SP-D contain predominantly dodecamers (four trimeric subunits), but also higher multimers have been observed. Each unit consists of at least four discrete structural domains: a short N-terminal domain; a relatively long collagenous domain, a short amphipathic connecting peptide, and a C-terminal, C- type lectin carbohydrate recognition domain (CRD). In the lungs, SP-D participates in the innate response to inhaled microorganisms and organic antigens. SP-D binds to surface glycoconjugates of various microorganisms and to oligosaccharides associated with the surface of numerous organic antigens. Studies have shown that SP-D expression may be related to a number of human diseases: cystic fibrosis, acute interstitial pneumonias (ARDS), asthma, bronchopulmonary dysplasia, alveolar proteinosis etc.

Features

• The total assay time is less than 5 hours
• The kit measures total surfactant protein D in serum, plasma (EDTA, citrate, heparin),

bronchoalveolar lavage fluid and amniotic fluid

• Assay format is 96 wells
• Quality Controls are human serum based. No animal sera are used
• Standards are recombinant protein based
• Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Amniotic fluid, Bronchoalveolar lavage, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

10 µl/well

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

1.56 to 100 ng/ml

Limit of detection

0.01 ng/ml

Intra-assay (Within-Run, n=8)

CV = 2.2 %

Inter-assay (Run-to-Run, n=5)

CV = 3.1 %

Spiking Recovery

93.2 %

Dilution Linearity

103.2 %

Cross-Reactivity

References to this product

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• Ulvestad B, Randem BG, Andersson L, Ellingsen DG, Barregard L. Clara cell protein as a biomarker for lung epithelial injury in asphalt workers. J Occup Environ Med. 2007 Oct;49 (10):1073-8
• Vaes B, Van Pottelbergh G, Mathei C, Legrand D, Olivier PA, Mekouar H, Rezzoug N, Wallemacq P, Pasquet A, Degryse J. The BELFRAIL (BFC80+) study: a population-based prospective cohort study of the very elderly in Belgium. BMC Geriatr. 2010;10:39
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References to summary

• Crouch EC. Surfactant protein-D and pulmonary host defense. Respir Res. 2000;1 (2):93-108
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• Honda Y, Kuroki Y, Matsuura E, Nagae H, Takahashi H, Akino T, Abe S. Pulmonary surfactant protein D in sera and bronchoalveolar lavage fluids. Am J Respir Crit Care Med. 1995 Dec;152 (6 Pt 1):1860-6
• Inoue T, Matsuura E, Nagata A, Ogasawara Y, Hattori A, Kuroki Y, Fujimoto S, Akino T. Enzyme-linked immunosorbent assay for human pulmonary surfactant protein D. J Immunol Methods. 1994 Aug 1;173 (2):157-64
• Nagae H, Takahashi H, Kuroki Y, Honda Y, Nagata A, Ogasawara Y, Abe S, Akino T. Enzyme-linked immunosorbent assay using F(ab')2 fragment for the detection of human pulmonary surfactant protein D in sera. Clin Chim Acta. 1997 Oct 31;266 (2):157-71
• Ohnishi H, Yokoyama A, Kondo K, Hamada H, Abe M, Nishimura K, Hiwada K, Kohno N. Comparative study of KL-6, surfactant protein-A, surfactant protein-D, and monocyte chemoattractant protein-1 as serum markers for interstitial lung diseases. Am J Respir Crit Care Med. 2002 Feb 1;165 (3):378-81
• Takahashi H, Fujishima T, Koba H, Murakami S, Kurokawa K, Shibuya Y, Shiratori M, Kuroki Y, Abe S. Serum surfactant proteins A and D as prognostic factors in idiopathic pulmonary fibrosis and their relationship to disease extent. Am J Respir Crit Care Med. 2000 Sep;162 (3 Pt 1):1109-14
• Takahashi H, Kuroki Y, Tanaka H, Saito T, Kurokawa K, Chiba H, Sagawa A, Nagae H, Abe S. Serum levels of surfactant proteins A and D are useful biomarkers for interstitial lung disease in patients with progressive systemic sclerosis. Am J Respir Crit Care Med. 2000 Jul;162 (1):258-63

By research topic:

• Immune Response, Infection and Inflammation

By molecule:

• Surfactant Protein D

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