sVE-Cadherin Monkey ELISA

Summary

VE-cadherin (about 130 kDa) belongs to the Cadherin adhesion molecules responsible for cellular interactions. VE-cadherin gene encodes a Ca2+-dependent cell adhesion molecule required for the organization of interendothelial junctions. This gene is expressed in endothelial cells.

Research topic

Animal studies, Cell adhesion proteins

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Baboon serum

Sample requirements

50 µl

Storage/Shipping

2–8°C/2–8°C, Std

Calibration Curve

Calibration range

0.19 – 12.5 U/ml

Limit of detection

0.10 U/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 10.0%

Spiking Recovery

80 %

Dilution Linearity

109 %

References to summary

• Alexander S, Willis BH, Xiao F, Robinson-Jackson S, Carden D, Moak C. Neutrophil elastase promotes lung microvascular injury and proteolysis of endothelial cadherins. Am J Physiol. 1998 Aug;275 (2 Pt 2):H385-92
• Ali J, Liao F, Martens E, Muller WA. Vascular endothelial cadherin (VE-cadherin): cloning and role in endothelial cell-cell adhesion. Microcirculation. 1997 Jun;4 (2):267-77
• Caveda L, Martin-Padura I, Navarro P, Breviario F, Corada M, Gulino D, Lampugnani MG, Dejana E. Inhibition of cultured cell growth by vascular endothelial cadherin (cadherin-5/VE-cadherin). J Clin Invest. 1996 Aug 15;98 (4):886-93
• Corada M, Caveda L, Martin-Padura I, Dejana E, Navarro P, Golay J, Breviario F, Introna M, Gulino D, Lampugnani MG. Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin. Arterioscler Thromb Vasc Biol. 1995 Aug;15 (8):1229-39
• Dejana E, Lampugnani MG, Mandoteanu I, Breviario F, Caveda L, Navarro P. Catenin-dependent and -independent functions of vascular endothelial cadherin. J Biol Chem. 1995 Dec 29;270 (52):30965-72
• Flamme I, Risau W. Vasculogenesis. Annu Rev Cell Dev Biol. 1995;11:73-91
• Geiger B, Ayalon O, Breviario F, Caveda L, Corada M, Lampugnani MG, Dejana E. The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin with vascular endothelial cadherin (VE-cadherin). J Cell Biol. 1995 Apr;129 (1):203-17
• Grant DS, Yaen CH, Busler D, Chalupowicz DG, Barsigian C, Bach TL, Martinez J. VE-Cadherin mediates endothelial cell capillary tube formation in fibrin and collagen gels. Exp Cell Res. 1998 Feb 1;238 (2):324-34
• Herren B, Levkau B, Raines EW, Ross R. Cleavage of beta-catenin and plakoglobin and shedding of VE-cadherin during endothelial apoptosis: evidence for a role for caspases and metalloproteinases. Mol Biol Cell. 1998 Jun;9 (6):1589-601
• Huber P, de Launoit Y, Kortulewski T, Prandini MH, Dalmon J, Gory S. Requirement of a GT box (Sp1 site) and two Ets binding sites for vascular endothelial cadherin gene transcription. J Biol Chem. 1998 Mar 20;273 (12):6750-5
• La Parola IL, Dejana E, Stoppacciaro A, Monardo F, Ruco LP, Uccini S, Cerimele D, Baroni CD. Co-expression of endothelial cell and macrophage antigens in Kaposi's sarcoma cells. J Pathol. 1994 May;173 (1):23-31
• Navarro P, Ruco L, Dejana E. Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization. J Cell Biol. 1998 Mar 23;140 (6):1475-84
• Risau W, Esser S, Lampugnani MG, Corada M, Dejana E. Vascular endothelial growth factor induces VE-cadherin tyrosine phosphorylation in endothelial cells. J Cell Sci. 1998 Jul;111 ( Pt 13):1853-65
• Ruco L, Dejana E, Martin-Padura I, De Castellarnau C, Uccini S, Pilozzi E, Nicotra MR, Natali PG, Ughi F, Azzolini C. Expression of VE (vascular endothelial)-cadherin and other endothelial-specific markers in haemangiomas. J Pathol. 1995 Jan;175 (1):51-7
• Smith ME, Jones TA, Hilton D. Vascular endothelial cadherin is expressed by perineurial cells of peripheral nerve. Histopathology. 1998 May;32 (5):411-3
• Suzuki S, St John T, Davidson M, Heimark RL, Obata S, Kido M, Tanihara H. Characterization of cadherin-4 and cadherin-5 reveals new aspects of cadherins. J Cell Sci. 1994 Jun;107 ( Pt 6):1697-704
• Tanihara H, Sano K, Heimark RL, St John T, Suzuki S. Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin. Cell Adhes Commun. 1994 Apr;2 (1):15-26
• Vestweber D, Mossmann H, Simon M, Boggemeyer E, Bosse R, Borges E, Gotsch U. VE-cadherin antibody accelerates neutrophil recruitment in vivo. J Cell Sci. 1997 Mar;110 ( Pt 5):583-8
• Vitolo D, Paradiso P, Uccini S, Ruco LP, Baroni CD. Expression of adhesion molecules and extracellular matrix proteins in glioblastomas: relation to angiogenesis and spread. Histopathology. 1996 Jun;28 (6):521-8

By research topic:

• Animal studies
• Cell adhesion proteins

By molecule:

• VE-Cadherin