sVEGF-R1/FLT-1 Human ELISA

Summary

Soluble VEGF-R1 is a naturally occurring endogenous form of the VEGF-R1 and was originally found in the supernatant of human vascular endothelial cells. It is generated by differential splicing of the flt-1 gene. In vitro sVEGF-R1 is used to inhibit VEGF-A mediated signals in endothelial cells, and in vivo it can be used to block physiological angiogenesis in several organs (e.g. ovary or bones).

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma-Citrate, Plasma-EDTA, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.16 – 10 ng/ml

Limit of detection

0.03 ng/ml

Intra-assay

CV = 5.1%

Inter-assay

CV = 5.4%

Spiking Recovery

102.7%

Dilution Linearity

102.8%.

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• VEGF receptor
• VEGF-R1/FLT-1

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