Testosterone ELISA

Summary

Testosterone is the most important male sex hormone, it is responsible for genital development, beard growth, muscle development and general male characteristics. The measurement of serum or plasma levels is an index of leydig cell function and high or low values correlate well with hypo or hyper gonadism. In females small amounts of testosterone are produced by the adrenals and ovaries. High levels of testosterone in females indicates excessive androgen production and are found in progressive hirsutism and virilization, Cushing’s syndrome and a deficiency in one or more of the specific enzymes required for normal steroid biosynthesis.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

50 μl

Storage/Shipping

2–8 °C

Calibration Curve

Calibration range

0.08 – 16 ng/ml

Limit of detection

0.022 ng/ml

References to summary

• Benraad TJ, Ross HA, Swinkels LM. A symmetric dialysis method for the determination of free testosterone in human plasma. Clin Chim Acta. 1987 Jun 15;165 (2-3):341-9
• Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Dechaud. H et al. Radioimmunoassay of testosterone not bound to sex hormone binding protein in plasma. Clin.Chem. 35: 1609 1989;
• Demers LM, Pardridge WM. Bioavailable testosterone in salivary glands. Clin Chem. 1991 Feb;37 (2):139-40
• Durnford R, Marcus GJ. A simple enzyme-linked immunosorbent assay for testosterone. Steroids. 1985 Dec;46 (6):975-86
• Ekins R. Hirsutism: free and bound testosterone. Ann Clin Biochem. 1990 Jan;27 ( Pt 1):91-4
• Joshi UM, Shah HP, Sudhama SP. A sensitive and specific enzymeimmunoassay for serum testosterone. Steroids. 1979 Jul;34 (1):35-46
• Noguchi M, Hirano T, Oka K. Changes in the concentration of testosterone in serum during the menstrual cycle, as determined by liquid chromatography. Clin Chem. 1988 Mar;34 (3):557-60
• Palatsi R, Hirvensalo E, Liukko P, Malmiharju T, Mattila L, Riihiluoma P, Ylostalo P. Serum total and unbound testosterone and sex hormone binding globulin (SHBG) in female acne patients treated with two different oral contraceptives. Acta Derm Venereol. 1984;64 (6):517-23
• Pandey HP, Rassaie MJ, Shrivastav TG, Kumari GL, Rao PN. Influence of different combinations of antibodies and penicillinase-labeled testosterone derivatives on sensitivity and specificity of immunoassays. Steroids. 1992 Mar;57 (3):112-8
• Santner SJ, Bardin CW, Nisula BC, Cefalu W, Pardridge WM, Manni A, Santen RJ. Bioavailability of albumin-bound testosterone. J Clin Endocrinol Metab. 1985 Oct;61 (4):705-10
• Sheldon PE, Coppenger CJ. A rapid radioimmunoassay for serum testosterone. Steroids. 1977 Aug;30 (2):149-57
• Singer F, Zumoff B. Subnormal serum testosterone levels in male internal medicine residents. Steroids. 1992 Feb;57 (2):86-9
• Swinkels, L. M. J. et al. Salivary and plasma free testosterone and androstenedione levels in women. Ann.Clin. Biochem. 25: 354, 1988;
• Tateiki, K., et al. Enzymeimmunoassay of serum Testosterone. Steroids. 30:25, 1977;
• Wilke TJ, Utley DJ. Total testosterone, free-androgen index, calculated free testosterone, and free testosterone by analog RIA compared in hirsute women and in otherwise-normal women with altered binding of sex-hormone-binding globulin. Clin Chem. 1987 Aug;33 (8):1372-5

By research topic:

• Steroid hormones

By molecule:

• Testosterone

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