TGF-beta1 Rat ELISA

Summary

TGF-beta belongs to a family of dimeric 25 kDa polypeptides that are ubiquitously distributed in tissues and synthesized by many different cells. TGF-β1 inhibits T- and B cell proliferation and inhibits maturation and activation of macrophages. It furthermore inhibits activity of natural killer cells and lymphokine activated killer cells and blocks production of cytokines.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

100 µl (pretreated)

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.48 – 30 ng/ml

Limit of detection

48 pg/ml

Intra-assay

CV = 5.4%

Inter-assay

CV = 10.6%

Spiking Recovery

88%

Dilution Linearity

117%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• TGF-beta1

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