TNF-alpha Mouse ELISA

Summary

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Research topic

Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

15.6 – 1000 pg/ml

Limit of detection

3 pg/ml

Intra-assay

CV = 4.8%

Inter-assay

CV = 4.4%

Spiking Recovery

97%

Dilution Linearity

94%

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• TNF-alpha

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