TNF-alpha Mouse ELISA, High Sensitivity

Summary

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Features

• Rapid assay (total reaction time: 4 hours.).
• A small sample volume (10µl in the standard procedure).
• An ecologically friendly preservative is used.
• Every reagent is provided in liquid form and ready to use.
• Excellent precision and reproducibility.

Research topic

Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma, Serum

Sample requirements

10 µl/well

Storage/Shipping

Store the kit at 2~8°C. Do not freeze.

Calibration Curve

Calibration range

16 – 3000 pg/ml

Spiking Recovery

CV = 100,1 %

References to this product

• Morimatsu F, Takahata Y, Sato M, Shimizu K, Kouguchi T, Zhang Y. Chicken collagen hydrolysate reduces proinflammatory cytokine production in C57BL/6.KOR-ApoEshl mice. J Nutr Sci Vitaminol (Tokyo). 2010;56 (3):208-10

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• TNF-alpha