TNF-alpha Rat ELISA, High Sensitivity

Summary

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Features

• Rapid assay (total reaction time: 4 hours.).
• A small sample volume (10μl in the standard procedure).
• An ecologically excellent preservative is used.
• Every reagent is provided in liquid form and ready to use.
• Excellent precision and reproducibility.

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma, Serum

Sample requirements

10 μl/well

Storage/Shipping

Store the kit at 2–8°C. Do not freeze.

Calibration Curve

Calibration range

16 – 3 000 pg/ml

Limit of detection

8 pg/ml

By research topic:

• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• TNF-alpha