TNF-beta Human ELISA

Summary

TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

100 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 1000 pg/ml

Limit of detection

4.6 pg/ml

Intra-assay

CV = 8.0%

Inter-assay

CV = 10%

Spiking Recovery

105 %

References to summary

• . A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
• . Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135 (3):2069-73
• . An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
• . Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
• . Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. 1984 Dec 20-1985 Jan 2;312 (5996):721-4
• . Disparate effects of tumor necrosis factor-alpha/cachectin and tumor necrosis factor-beta/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol. 1987 Jun 15;138 (12):4298-302
• . Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J Exp Med. 1986 Mar 1;163 (3):632-43
• . Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A. 1986 Nov;83 (22):8699-702
• . Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta). J Immunol Methods. 1990 Aug 7;131 (2):283-9
• . Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
• . Increased lymphotoxin in human malarial serum, and the ability of this cytokine to increase plasma interleukin-6 and cause hypoglycaemia in mice: implications for malarial pathology. Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86 (6):602-7
• . Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas. Proc Natl Acad Sci U S A. 1992 Nov 1;89 (21):10036-40
• . Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
• . Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
• . Optimal collection of blood samples for the measurement of tumor necrosis factor alpha. Cytokine. 1990 Sep;2 (5):353-6
• . Serum-free in vitro bioassay for the detection of tumor necrosis factor. J Immunol Methods. 1986 Nov 6;93 (2):201-6
• . Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6-12;319 (6053):516-8
• . The divergent receptors for TNF. Trends Biochem Sci. 1990 Oct;15 (10):366-8
• . Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
• . Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia. Blood. 1991 Jun 1;77 (11):2451-5
• . Tumor necrosis factor-beta induces sleep, fever, and anorexia. Am J Physiol. 1992 Sep;263 (3 Pt 2):R703-7
• . Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• . Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• . Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons. Nature. 1986 Oct 30-Nov 5;323 (6091):819-22
• Adolf GR, Apfler I. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
• Bertolini DR, Nedwin GE, Bringman TS, Smith DD, Mundy GR. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6-12;319 (6053):516-8
• Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
• Broudy VC, Harlan JM, Adamson JW. Disparate effects of tumor necrosis factor-alpha/cachectin and tumor necrosis factor-beta/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol. 1987 Jun 15;138 (12):4298-302
• Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
• Clark IA, Gray KM, Rockett EJ, Cowden WB, Rockett KA, Ferrante A, Aggarwal BB. Increased lymphotoxin in human malarial serum, and the ability of this cytokine to increase plasma interleukin-6 and cause hypoglycaemia in mice: implications for malarial pathology. Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86 (6):602-7
• Exley AR, Cohen J. Optimal collection of blood samples for the measurement of tumor necrosis factor alpha. Cytokine. 1990 Sep;2 (5):353-6
• Gray PW, Aggarwal BB, Benton CV, Bringman TS, Henzel WJ, Jarrett JA, Leung DW, Moffat B, Ng P, Svedersky LP, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. 1984 Dec 20-1985 Jan 2;312 (5996):721-4
• Himmler A, Maurer-Fogy I, Kronke M, Scheurich P, Pfizenmaier K, Lantz M, Olsson I, Hauptmann R, Stratowa C, Adolf GR. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
• Ishibashi K, Ishitsuka K, Chuman Y, Otsuka M, Kuwazuru Y, Iwahashi M, Utsunomiya A, Hanada S, Sakurami T, Arima T. Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia. Blood. 1991 Jun 1;77 (11):2451-5
• Kapas L, Krueger JM. Tumor necrosis factor-beta induces sleep, fever, and anorexia. Am J Physiol. 1992 Sep;263 (3 Pt 2):R703-7
• Kramer SM, Carver ME. Serum-free in vitro bioassay for the detection of tumor necrosis factor. J Immunol Methods. 1986 Nov 6;93 (2):201-6
• Lamche HR, Adolf GR. Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta). J Immunol Methods. 1990 Aug 7;131 (2):283-9
• Locksley RM, Heinzel FP, Shepard HM, Agosti J, Eessalu TE, Aggarwal BB, Harlan JM. Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• Locksley RM, Heinzel FP, Shepard HM, Agosti J, Eessalu TE, Aggarwal BB, Harlan JM. Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• Old LJ. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
• Paul NL, Ruddle NH. Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
• Picarella DE, Kratz A, Li CB, Ruddle NH, Flavell RA. Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas. Proc Natl Acad Sci U S A. 1992 Nov 1;89 (21):10036-40
• Scheurich P, Thoma B, Ucer U, Pfizenmaier K. Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
• Shalaby MR, Aggarwal BB, Rinderknecht E, Svedersky LP, Finkle BS, Palladino MA Jr. Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135 (3):2069-73
• Spies T, Morton CC, Nedospasov SA, Fiers W, Pious D, Strominger JL. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A. 1986 Nov;83 (22):8699-702
• Sprang SR. The divergent receptors for TNF. Trends Biochem Sci. 1990 Oct;15 (10):366-8
• Vilcek J, Palombella VJ, Henriksen-DeStefano D, Swenson C, Feinman R, Hirai M, Tsujimoto M. Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J Exp Med. 1986 Mar 1;163 (3):632-43
• Wong GH, Goeddel DV. Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons. Nature. 1986 Oct 30-Nov 5;323 (6091):819-22

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• TNF-beta

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