TNF-beta Human ELISA

Summary

TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells.

Research topic

Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

100 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 1000 pg/ml

Limit of detection

4.6 pg/ml

Intra-assay

CV = 8.0%

Inter-assay

CV = 10%

Spiking Recovery

105 %

References to summary

• . A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
• . Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135 (3):2069-73
• . An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
• . Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
• . Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. 1984 Dec 20-1985 Jan 2;312 (5996):721-4
• . Disparate effects of tumor necrosis factor-alpha/cachectin and tumor necrosis factor-beta/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol. 1987 Jun 15;138 (12):4298-302
• . Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J Exp Med. 1986 Mar 1;163 (3):632-43
• . Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A. 1986 Nov;83 (22):8699-702
• . Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta). J Immunol Methods. 1990 Aug 7;131 (2):283-9
• . Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
• . Increased lymphotoxin in human malarial serum, and the ability of this cytokine to increase plasma interleukin-6 and cause hypoglycaemia in mice: implications for malarial pathology. Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86 (6):602-7
• . Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas. Proc Natl Acad Sci U S A. 1992 Nov 1;89 (21):10036-40
• . Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
• . Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
• . Optimal collection of blood samples for the measurement of tumor necrosis factor alpha. Cytokine. 1990 Sep;2 (5):353-6
• . Serum-free in vitro bioassay for the detection of tumor necrosis factor. J Immunol Methods. 1986 Nov 6;93 (2):201-6
• . Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6-12;319 (6053):516-8
• . The divergent receptors for TNF. Trends Biochem Sci. 1990 Oct;15 (10):366-8
• . Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
• . Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia. Blood. 1991 Jun 1;77 (11):2451-5
• . Tumor necrosis factor-beta induces sleep, fever, and anorexia. Am J Physiol. 1992 Sep;263 (3 Pt 2):R703-7
• . Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• . Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• . Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons. Nature. 1986 Oct 30-Nov 5;323 (6091):819-22
• Adamson JW, Harlan JM, Broudy VC. Disparate effects of tumor necrosis factor-alpha/cachectin and tumor necrosis factor-beta/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol. 1987 Jun 15;138 (12):4298-302
• Adolf GR, Lamche HR. Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta). J Immunol Methods. 1990 Aug 7;131 (2):283-9
• Adolf GR, Stratowa C, Hauptmann R, Olsson I, Lantz M, Pfizenmaier K, Scheurich P, Kronke M, Maurer-Fogy I, Himmler A. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
• Aggarwal BB, Ferrante A, Rockett KA, Cowden WB, Rockett EJ, Gray KM, Clark IA. Increased lymphotoxin in human malarial serum, and the ability of this cytokine to increase plasma interleukin-6 and cause hypoglycaemia in mice: implications for malarial pathology. Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86 (6):602-7
• Apfler I, Adolf GR. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
• Arima T, Sakurami T, Hanada S, Utsunomiya A, Iwahashi M, Kuwazuru Y, Otsuka M, Chuman Y, Ishitsuka K, Ishibashi K. Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia. Blood. 1991 Jun 1;77 (11):2451-5
• Carver ME, Kramer SM. Serum-free in vitro bioassay for the detection of tumor necrosis factor. J Immunol Methods. 1986 Nov 6;93 (2):201-6
• Cerami A, Beutler B. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
• Cohen J, Exley AR. Optimal collection of blood samples for the measurement of tumor necrosis factor alpha. Cytokine. 1990 Sep;2 (5):353-6
• et al., Svedersky LP, Ng P, Moffat B, Leung DW, Jarrett JA, Henzel WJ, Bringman TS, Benton CV, Aggarwal BB, Gray PW. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature. 1984 Dec 20-1985 Jan 2;312 (5996):721-4
• Flavell RA, Ruddle NH, Li CB, Kratz A, Picarella DE. Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas. Proc Natl Acad Sci U S A. 1992 Nov 1;89 (21):10036-40
• Goeddel DV, Wong GH. Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons. Nature. 1986 Oct 30-Nov 5;323 (6091):819-22
• Harlan JM, Aggarwal BB, Eessalu TE, Agosti J, Shepard HM, Heinzel FP, Locksley RM. Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• Harlan JM, Aggarwal BB, Eessalu TE, Agosti J, Shepard HM, Heinzel FP, Locksley RM. Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J Immunol. 1987 Sep 15;139 (6):1891-5
• Krueger JM, Kapas L. Tumor necrosis factor-beta induces sleep, fever, and anorexia. Am J Physiol. 1992 Sep;263 (3 Pt 2):R703-7
• Mundy GR, Smith DD, Bringman TS, Nedwin GE, Bertolini DR. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6-12;319 (6053):516-8
• Old LJ. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
• Palladino MA Jr, Finkle BS, Svedersky LP, Rinderknecht E, Aggarwal BB, Shalaby MR. Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135 (3):2069-73
• Pfizenmaier K, Ucer U, Thoma B, Scheurich P. Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
• Ruddle NH, Paul NL. Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
• Sprang SR. The divergent receptors for TNF. Trends Biochem Sci. 1990 Oct;15 (10):366-8
• Strominger JL, Pious D, Fiers W, Nedospasov SA, Morton CC, Spies T. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A. 1986 Nov;83 (22):8699-702
• Tsujimoto M, Hirai M, Feinman R, Swenson C, Henriksen-DeStefano D, Palombella VJ, Vilcek J. Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J Exp Med. 1986 Mar 1;163 (3):632-43
• Williamson B, Fiore N, Green S, Kassel RL, Old LJ, Carswell EA. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• TNF-beta

Registration form