Troponin I Human ELISA

Summary

Troponin I consists of 181–211 amino acid residues, and the cardiac isoform is larger due to the presence of an additional N-terminal peptide which has a biologically important function in the interactions of troponin I and troponin C. The main function of troponin I is the inhibition of actomyosin ATPase activity. Cardiac troponin I and T are sensitive biomarkers of myocardial injury and have become central to the diagnosis of myocardial infarction. They are elevated in many clinical syndromes associated with direct myocardial injury, myocardial ischemia, or ventricular strain. They are also released in a number of clinical situations in which thrombotic complications of coronary artery disease. These situations include conditions like pulmonary embolism, sepsis, myocarditis, and acute stroke.

Research topic

Cardiovascular disease

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell lysate, Plasma, Serum

Storage/Shipping

All kit components of this kit are stable at 2 to 8°C. Any unused reconstituted standard should be discarded or frozen at –70°C. Standard can be frozen and thawed one time only without loss of immunoreactivity.

Calibration Curve

Calibration range

0–25 ng/ml

Limit of detection

0.38 ng/ml

Intra-assay (Within-Run, n=6)

CV = 6.1%

Inter-assay (Run-to-Run, n=3)

CV = 2.8%

By research topic:

• Cardiovascular disease

By molecule:

• Troponin I