TSH Receptor Autoantibody Human ELISA 2. generation

Summary

Hyperthyroidism in Graves’ disease is due to the presence of autoantibodies to TSHR and measurement of these autoantibodies can be useful in disease diagnosis and management.

Research topic

Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample requirements

100 μl

Calibration Curve

Calibration range

1 – 40 U/l

Limit of detection

0.21 U/ml

References to summary

• Bolton J, Sanders J, Oda Y, Chapman C, Konno R, Furmaniak J, Rees Smith B. Measurement of thyroid-stimulating hormone receptor autoantibodies by ELISA. Clin Chem. 1999 Dec;45 (12):2285-7
• Kamijo K. TSH-receptor antibody measurement in patients with various thyrotoxicosis and Hashimoto's thyroiditis: a comparison of two two-step assays, coated plate ELISA using porcine TSH-receptor and coated tube radioassay using human recombinant TSH-receptor. Endocr J. 2003 Feb;50 (1):113-6
• Smith BR, Bolton J, Young S, Collyer A, Weeden A, Bradbury J, Weightman D, Perros P, Sanders J, Furmaniak J. A new assay for thyrotropin receptor autoantibodies. Thyroid. 2004 Oct;14 (10):830-5

By research topic:

• Autoimmunity

By molecule:

• TSH Receptor Autoantibody

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