TSH Receptor Autoantibody Human ELISA 3. generation

Summary

Hyperthyroidism in Graves’ disease is due to the presence of autoantibodies to TSHR and measurement of these autoantibodies can be useful in disease diagnosis and management.

Research topic

Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Calibration Curve

Calibration range

0.4 to 30 U/l

Limit of detection

0.08 U/l

Intra-assay

CV = 4.9 %

Inter-assay

CV = 8.8%

References to summary

• Kamijo K, Ishikawa K, Tanaka M. Clinical evaluation of 3rd generation assay for thyrotropin receptor antibodies: the M22-biotin-based ELISA initiated by Smith. Endocr J. 2005 Oct;52 (5):525-9
• Smith BR, Bolton J, Young S, Collyer A, Weeden A, Bradbury J, Weightman D, Perros P, Sanders J, Furmaniak J. A new assay for thyrotropin receptor autoantibodies. Thyroid. 2004 Oct;14 (10):830-5

By research topic:

• Autoimmunity

By molecule:

• TSH Receptor Autoantibody

Registration form