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Tumor Necrosis Factor-alpha Human ELISA

Other names: TNF-α, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor-alpha Product of BioVendor
Product: Size:
New: RBMS223/4R (regulatory status: RUO) 96 wells (1 kit)
Files: Datasheet PDF (RUO)

Product details


Summary

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Research topic

Cytokines and chemokines and related molecules


Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine

Sample requirements

50 µl/well

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 500 pg/ml

Limit of detection

2.3 pg/ml

Intra-assay

6.0%

Inter-assay

7.4%



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