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Products\Immunoassays\Tumor Necrosis Factor-alpha Human ELISA:
Tumor Necrosis Factor-alpha Human ELISA
| Other names: TNF-α, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor-alpha | Product of BioVendor | ||||
| Product: | Size: | ||||
|---|---|---|---|---|---|
| New: RBMS223/4R (regulatory status: RUO) | 96 wells (1 kit) | ||||
| Files: Datasheet PDF (RUO) | |||||
Product details
Summary
TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccharides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.
Research topic
Cytokines and chemokines and related molecules
Assay format
Sandwich ELISA, Biotin-labelled antibody
Applications
Cell culture supernatant, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine
Sample requirements
50 µl/well
Storage/Shipping
2–8°C
Calibration Curve
|
Calibration range
7.8 – 500 pg/ml
Limit of detection
2.3 pg/ml
Intra-assay
6.0%
Inter-assay
7.4%
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