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Products\Immunoassays\Tumor Necrosis Factor-alpha Human ELISA:
Tumor Necrosis Factor-alpha Human ELISA
| Other names: | TNF-α, Cachectin, Tumor necrosis factor ligand superfamily member 2 | ||
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| Cat. No.: | ROTNFA021R | Regulatory status: RUO | |
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| Files: | Datasheet PDF (RUO) MSDS (RUO) | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Research topic
Cytokines and Chemokines and related molecules
Storage/Shipping
Store at 2–8°C
Summary
TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytototic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial lipopolysaccharides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.
Assay format
Sandwich ELISA, HRP-labelled antibody
Sample requirements
50 µl/well
Applications
Cell culture medium, Cerebrospinal fluid, Fluids, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine
Calibration Curve
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Calibration range
3.9 to 250 pg/ml
Limit of detection
<4 pg/ml
Intra-assay
<6%
Inter-assay
<4%
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