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Products\Immunoassays\Tumor Necrosis Factor-alpha Mouse ELISA:
Tumor Necrosis Factor-alpha Mouse ELISA
| Other names: | TNF-α, Cachectin, Tumor necrosis factor ligand superfamily member 2 | ||
|---|---|---|---|
| Cat. No.: | RSHAKMTN011R | Regulatory status: RUO | |
| Size: | 96 wells (1 kit) | | | |
| Files: | Datasheet PDF (RUO) | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
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Research topic
Animal studies, Cytokines and chemokines and related molecules
Features
- Rapid assay (total reaction time: 4 hours.).
- A small sample volume (10µl in the standard procedure).
- An ecologically friendly preservative is used.
- Every reagent is provided in liquid form and ready to use.
- Excellent precision and reproducibility.
Storage/Shipping
Store the kit at 2~8°C. Do not freeze.
Summary
TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccharides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.
Assay format
Sandwich ELISA, Biotin-labelled antibody
Sample requirements
10 µl/well
Calibration range
16 – 3000 pg/ml
Spiking Recovery
CV = 100,1 %
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