VEGF Human ELISA

Research topic

Cytokines and Chemokines and related molecules

Storage/Shipping

+4°C/ Wet ice

Summary

VEGF is characterized by its highly specific mitogenic activity for endothelial cells and its angiogenic effect observed in vitro and in vivo. The VEGF family consists of four isoforms (A-D). VEGF-A is considered the most important of these with respect to tumor angiogenesis, especially the splice variant isoforms VEGF121 and VEGF165 were found to be secreted by a wide spectrum of cell types, including smooth muscle cells, fibroblasts and epithelial cells keratinocytes macrophages, cardiac myocytes and various tumor cells. Inhibition of VEGF with a specific monoclonal antibody can suppress tumor growth in vivo.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

20 µl/well

Applications

Cell culture medium, Cerebrospinal fluid, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine

Calibration Curve

Calibration range

25 to 6 000 pg/ml

Limit of detection

<25 pg/ml

Intra-assay

<6%

Inter-assay

<10%

References to summary

• Ferrara, N., Houck, K., Jakeman, L., and Leung, D.W.: Molecular and biological properties of the vascular endothelial growth factor family of proteins.Endocr. Rev. 13, 18–32 (1992).
• Ferrara N.: Timeline: VEGF and the quest for tumour angiogenesis factors.Nat Rev Cancer 2, 795–803 (2002).
• Tischer, E., Mitchell, R., Hartmann, T., Silva, M., Gospodarowicz, D., Fiddes, J.C., and Abraham, J.A.: The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing.J. Biol. Chem. 266, 11947–11954 (1991).
• Ferrara, N., Winer, J., and Burton, T.: Aortic smooth muscle cells express and secrete vascular endothelial growth factor.Growth Factors 5, 141–148 (1991).
• Pertovaara, L., Kaipainen, A., Mustonen, T., Orpana, A., Ferrara, N., Saksela, O., and Alitalo, K.: Vascular endothelial growth factor is induced in response to transforming growth factor-b in fibroblastic and epithelial cells.J. Biol. Chem. 269, 6271–6274 (1994).
• Brown, L.F., Yeo, K.-T., Berse, B., Yeo, T.K., Senger, D.R., Dvorak, H.F., and van de Water, L.: Expression of vascular permeability factor (vascular endothelial growth factor) by epidermal keratinocytes during wound healing.J. Exp. Med. 176, 1375–1379 (1992).
• Berse, B., Brown, L.F., Van de Water, L., Dvorak, H.F., and Senger, D.R. : Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages, and tumors.Am. J. Pathol. 3, 211–220 (1992).
• Ladoux, A., and Frelin, C.: Hypoxia is a strong inducer of vascular endothelial growth factor mRNA expression in the heart. Biochem. Biophys.Res. Commun. 195, 1005–1010 (1993).