Bone Morphogenetic Protein-2 Human, Recombinant protein

Introduction to the Molecule

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Description

Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. The BMP-2 is purified by proprietary chromatographic techniques.

Amino Acid Sequence

Source

E. coli

Purity

Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Biological Activity

1. The ED50 as determined by the cytolysis of MC3T3-E1 cells is < 50 ng/ml.
2. When implanted in the mouse thighbone muscle and grow for 14 days, Muscle tissue was harvested and examined biochemically for calcium uptake. Our unit defined as 1 microgram calcium absorbed. The specific activity is more than 1000 Units/mg.
3. The activity of BMP-2 was tested using alkaline phosphatase, the activity in mouse muscle myoblast C2C12 cell line was found to be 1×105 U/mg protein.
4. The ectopic osteogenesis capacity is 1000mg fresh bone per 1mg of BMP-2.

Formulation

BMP-2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM NaAc pH=4.

Storage, Stability/Shelf Life

Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution BMP2 should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder

By molecule:

• Bone Morphogenetic Protein-2 (BMP-2)