Bone Morphogenetic Protein 7 Human, Recombinant protein

Description

N-TERMINAL---Human BMP-2 (Met 1 – Arg 282) Human BMP-7 (Ser 293 – Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40 kDa in SDS-PAGE under non-reducing conditions. BMP-7 is purified by proprietary chromatographic techniques.

Source

Chinese Hamster Ovary Cells (CHO)

Purity

Greater than 97.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Biological Activity

Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is < 100 ng/mL.

Formulation

BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine, and 0.005% tween 20 pH-4.

Storage, Stability/Shelf Life

Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

By molecule:

• Bone Morphogenetic Protein 7