Complement C1q tumor necrosis factor-related protein 1 Human E. coli

Research topic

Cardiovascular disease, Energy metabolism and body weight regulation

Description

Total 266 AA. MW: 30.45 kDa (calculated). N-terminal His-tag, 10extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

Purity

>95%

SDS-PAGE gel

12% SDS-PAGE separation of Human CTRP1 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5μg / lane 3. non-reduced and non-heated sample, 5μg / lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH 4.0

Reconstitution

Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

ELISA, Western blotting

Note

This product is intended for research use only.

References

• Davis KE, Scherer PE. Adiponectin: no longer the lone soul in the fight against insulin resistance?. Biochem J. 2008 Dec 1;416 (2):e7-9
• Klemmer PJ, Bomback AS. Interaction of aldosterone and extracellular volume in the pathogenesis of obesity-associated kidney disease: a narrative review. Am J Nephrol. 2009;30 (2):140-6
• Lasser G, Guchhait P, Ellsworth JL, Sheppard P, Lewis K, Bishop P, Cruz MA, Lopez JA, Fruebis J. C1qTNF-related protein-1 (CTRP-1): a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking VWF binding to collagen. Blood. 2006 Jan 15;107 (2):423-30
• Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions. Biochem J. 2008 Dec 1;416 (2):161-77
• Yang Y, Kim E, Oh GT, Kim SH, Yoon do Y, Kim KI, Lim JS, Han SH, Kim D, Kim JW, Lee YH, Cho H, Baek A, Kim JH, Kim KY, Jeon JH. A novel adipokine CTRP1 stimulates aldosterone production. FASEB J. 2008 May;22 (5):1502-11
• Yang Y, Yoon DY, Kim KI, Yoon S, Lee MS, Cho DH, Lim JS, Han SH, Lee YH, Kim DH, Lee CH, Kim JH, Kim HY, Kim KY. Tumor necrosis factor-alpha and interleukin-1beta increases CTRP1 expression in adipose tissue. FEBS Lett. 2006 Jul 10;580 (16):3953-60

By research topic:

• Cardiovascular disease
• Energy metabolism and body weight regulation

By molecule:

• Complement C1q tumor necrosis factor-related protein 1

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