Complement C1q tumor necrosis factor-related protein 8 Human E. coli

Description

Total 255 AA. MW: 28,4 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

SDS-PAGE gel

14% SDS-PAGE separation of Human CTRP8 reduced and heated sample, 5μg / lane M.W. marker – 14, 21, 31, 45, 66, 97 kDa non-reduced and non-heated sample, 5μg / lane

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 0.03M Acetate buffer, pH 4

Reconstitution

Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Western blotting

By molecule:

• Complement C1q tumor necrosis factor-related protein 8