Corticotropin-Releasing Factor-Binding Protein Human E. coli

Research topic

Steroid hormones

Description

Total 308 AA. MW: 34.58 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

SDS-PAGE gel

14% SDS-PAGE separation of Human CRFBP 1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa 2. reduced and boiled sample, 2μg/lane 3. non-reduced and non-boiled sample, 2μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

ELISA, Western blotting

By research topic:

• Steroid hormones

By molecule:

• Corticotropin-Releasing Factor-Binding Protein