CXCL12 (isoform alpha) Human E.coli

Research topic

Cytokines and chemokines and related molecules, Immune Response, Infection and Inflammation

Description

Total 78 AA. MW: 9.2 kDa (calculated). N-terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

Purity

>95%

SDS-PAGE gel

12% SDS-PAGE separation of Human CXCL12 M.W. marker – 14, 21, 31, 45, 66, 97 kDa reduced and heated sample, 5μg / lane non-reduced and non-heated sample, 5μg / lane

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 20mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Western blotting

Note

This product is intended for research use only.

By research topic:

• Cytokines and chemokines and related molecules
• Immune Response, Infection and Inflammation

By molecule:

• CXCL12