Endostatin Human

Introduction to the Molecule

Endostatin is a 20 kDa C-terminal fragment of collagen XVIII. The exact molecular mass determined by ESI-MS was found to be 18 494 Da.Endostatin specifically inhibits. endothelial proliferation and potently inhibits angiogenesis and tumor growth. Anti-endostatin monoclonal antibody administered to nude mice transplanted with human hepatocellular carcinoma cells (JHH-1 line) that endogenously produced endostatin promoted tumor angiogenesis by inhibiting endostatin activity in the tumor and subsequently increased tumor mass by preventing cancer cells from undergoing apoptosis. The ability of endostatin to bind Zn2+ is essential for its antiangiogenic activity. The endostatin precursor collagen XVIII is expressed at high levels in human livers, the main source being hepatocytes. The generation of endostatin or endostatin-like collagen XVIII fragments is catalyzed by proteolytic enzymes, including cathepsin L and matrix metalloproteases, that cleave peptide bonds within the protease-sensitive hinge region of the C-terminal domain. The processing of collagen XVIII to endostatin may represent a local control mechanism for the regulation of angiogenesis. Circulating endostatin was detected in patients with various types of cancer as well as in healthy controls. Circulating endostatin concentrations are significantly increased in patients with systemic sclerosis. Interestingly, pericardial fluid levels of endostatin are associated with the presence (40% lower levels) or absence of collaterals in patients with CAD.

Research topic

Oncology

Description

Endostatin Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide having a total molecular mass of 20,000 Dalton C-terminal fragment of collagen XVIII that has been shown to act as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. Induces tyrosine phosphorylation of Shc (SH2 domain adapter protein) leading to specific inhibition in endothelial cell prolifiration. The Endostatin is purified by proprietary chromatographic techniques.

Amino Acid Sequence

Source

Pichia Pastoris

Purity

Greater than 98.0% as determined by:

• (a) Analysis by RP-HPLC.
• (b) Analysis by SDS-PAGE.

Biological Activity

The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.

Formulation

The recombinant Human Endostatin is formulated with PBS.

Storage, Stability/Shelf Life

Endostatin although stable at 4°C for 30 days, shou ld be stored desiccated below –20°C for periods greater than 30 days. Please avoid Freeze/Thaw cycles.

Physical Appearance

Sterile filtered liquid formulation.

By research topic:

• Oncology

By molecule:

• Endostatin