Fetuin-B Human HEK293

Introduction to the Molecule

Fetuin B is a member of cystatin superfamily proteins, comprises two cystatin domains. It was found in secreted form in serum, it is expressed in liver, tongue and placenta. It regulates osteogenesis by inhibition effect on mineralization. It plays a role in oocyte maturation.

Description

MW: 41.8 kDa (calculated), migrates at ~ 55 kDa on SDS-PAGE; N-terminal linker (4 extra AA highlighted), C-terminal His-tag (6 extra AA highlighted). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

Source

HEK293

Purity

Purity as determined by densitometric image analysis: >90%

SDS-PAGE gel

12% SDS-PAGE separation of Human Fetuin-B M.W. marker – 97, 66, 45, 31, 21, 14 kDa reduced and boiled sample, 5μg / lane non-reduced and non-boiled sample, 5μg / lane

Endotoxin

<1.0 EU/µg

Formulation

Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL TEST to determine quantity of endotoxin.

Applications

Cell culture and/or animal studies, ELISA, Western blotting

Note

This product is intended for research use only. The Certificate of Analysis is available on www.biovendor.com

References

• Denecke B, Graber S, Schafer C, Heiss A, Woltje M, Jahnen-Dechent W. Tissue distribution and activity testing suggest a similar but not identical function of fetuin-B and fetuin-A. Biochem J. 2003 Nov 15;376 (Pt 1):135-45
• Schroeder AC, Schultz RM, Kopf GS, Taylor FR, Becker RB, Eppig JJ. Fetuin inhibits zona pellucida hardening and conversion of ZP2 to ZP2f during spontaneous mouse oocyte maturation in vitro in the absence of serum. Biol Reprod. 1990 Nov;43 (5):891-7

By molecule:

• Fetuin-B

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