Fibronectin, Human purified protein

Introduction to the Molecule

Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis. Fibronectin is secreted as a dimer with a monomer molecular weight of 220–250 kDa. It is synthesized by, and present around, fibroblasts, endothelial cells, chondrocytes, glial cells, and myocytes. Fibronectin´s im­portance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation.

Research topic

Oncology, Transplantation

Description

Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE. Fibronectin plays a role in several cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the etracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin alos takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound Fibronectin receptors on cell surfaces.

Source

Human plasma

Purity

Greater than 95.0% as determined by analysis by SDS-PAGE.

Formulation

The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris HCl pH-7.5 and 0.1M NaCl.

Storage, Stability/Shelf Life

Lyophilized Fibronectin although stable at room temperature for 1 week, should be stored desiccated below –18°C. Upon reconstitution Fibronectin should be stored at 4°C between 2–7 days and for future use below –18°C. Please prevent freeze-thaw cycles.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

By research topic:

• Oncology
• Transplantation

By molecule:

• Fibronectin