Galactoside-Binding Soluble Lectin 13 Human E. coli

Research topic

Reproduction

Description

Total 149 AA. MW: 17.36 kDa (calculated). N-Terminal His-tag (10 extra AA)

Amino Acid Sequence

Source

E. coli

Purity

>90%

SDS-PAGE gel

14% SDS-PAGE separation of Human PP13 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and boiled sample, 5μg/lane 3. non-reduced and non-boiled sample, 5μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week. Avoid storage of lyophilized product at room temperature. Transport on dry ice.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL TEST to determine endotoxin level.

Applications

ELISA, Immunoassays, Western blotting

By research topic:

• Reproduction

By molecule:

• Galactoside-Binding Soluble Lectin 13